Research the Cytotoxicity of Docetaxel Combined with Carboplatin in Osteosarcoma Cell Line MG-63
Abstract: Objective: To research the cytotoxicity of docetaxel combined with carboplatin on osteosarcoma cell line MG-63. Methods: Using MTT assay, we obtained the mean IC50 of docetaxel and caboplatin for the osteosarcoma cell line MG-63. The MG-63 was incubated with IC50 docetaxel, IC50 carboplatin and 1/2 IC50 docetaxel + 1/2 IC50 carboplatin for 24 h, 48 h, 72 h. The cytotoxicity of each group was measured by MTT assays. Result: By the formula IC50 = lg − 1[Xm − i(ΣP − 0.5)] to obtain the IC50 of docetaxel and carboplatin for osteosarcoma cell MG-63 were 0.25 mg/ml and 4.00 mg/ml respectively. The mean cytotoxicity index (CI) of 0.25 mg/ml docetaxel, 2 mg/ml carboplatin and 0.125 mg/ml docetaxel + 2 mg/ml carboplatin for 24 h were 38.6%, 48.7% and 60.0% respectively (P < 0.05); and those for 48 h were 44.3%, 49.2% and 73.0% respectively (P < 0.05); and those for 72 h were 59.3%, 58.6% and 79.9% respectively (the 0.25 mg/ml docetaxel group and 2 mg/ml carboplatin group P > 0.05; the combined group compared with the other two groups P < 0.05). The cytotoxicity index of combined group was significant increased. Conclusion: The combination of half dose docetaxel with carboplatin appears more active as docetaxel or carboplatin alone in full doses in treatment of osteosarcoma cell line MG-63 in vitro.
文章引用: 谭泽恩 , 董乐乐 (2015) 多西紫杉醇协同卡铂对骨肉瘤细胞MG-63细胞毒性的实验研究。 临床医学进展， 5， 43-49. doi: 10.12677/ACM.2015.51009
 Link, M.P., Coorin, A.M., Miser, A.W., et al. (1986) The effect of adjuvant chemotherapy on relanse-free survival in patients with osreosarcoma of the extremity. New England Journal of Medicine, 314, 1600.
 陈伟, 董乐乐 (2009) 骨肉瘤治疗进展. 包头医学院学报, 5, 108-110.
 彭磊, 王臻等 (2001) 多西紫杉醇诱导骨肉瘤凋亡的实验研究. 中华肿瘤杂志, 3, 14-16.
 Fabbri, F., Carloni, S., Brigliadori, G., et al. (2006) Sequential events of apoptosis involving docetaxel, a microtubule- inferfering agent: A cytometric study. BMC Cell Biology, 7, 6.
 曾洵 (2008) 多西紫杉醇及联合卡铂对人视网膜母细胞瘤株SO-RB50的增殖抑制. 昆明医学院, 昆明.
 吕星, 郭翔, 洪明晃, 陈秋燕, 曾奇, 向燕群 (2010) 多西紫杉醇联合卡铂与氟尿嘧啶联合卡铂治疗局部晚期鼻咽癌近期疗效的比较. 癌症, 2, 148-152.