A Study on Bacteriostatic Activity of Bacteriocins Produced by Probiotic Bacteria
Abstract: Object: To reveal the bacteriostatic activity of bacteriocins produced by human intestinal probiotics. Methods: Intestinal probiotics were separated from excrement of healthy people and identified by routine method and PCR. Bacteriocins were refined as Yang’s way and strains that could produce broad-spectrum efficient bacteriocins were selected by using 96-well plate method. Bacteriostatic activity of every selected bacteriocin was detected on S. aureus, S. pneumoniae, E. coli, K. pneumonia, P. vulgaris, S. typhi, S. flexneri, P. aeruginosa, A. baumanni and C. albicans by 96-well plate method respectively. Results: 32 strains of intestinal probiotics were separated, including 4 bifidobacteria (B), 6 lactobacillus (L), and 22 enterococcus (E). B3, B4, L5, E12, E15, E18 were broad-spectrum efficient bacteriocin producing strains. Bacteriocin of B3, L5, E15 has bacteriostatic action on gram positive and negative bacteria. Both B3 and L5 bacteriocin has strong inhibitory action on S. typhi and S. pneumoniae, while E15’ shows higher inhibitory effects on C. albicans and S. pneumoniae than other bacteria used in this study. Conclusion: Bacteriocins produced by intestinal probiotic bacteria have broad bacteriostatic activity, and some of them are high efficient.
文章引用: 王 澜 , 周 燕 , 吴碧婷 , 李海燕 , 杜季梅 (2015) 益生菌细菌素的抑菌活性研究。 医学诊断， 5， 6-11. doi: 10.12677/MD.2015.51002
 樊亭亭 (2012) 我国抗生素滥用规制研究. 硕士论文, 南京中医药大学, 南京.
 丁成为 (2007) 乳酸片球菌素抑菌谱及应用性研究. 硕士论文, 天津大学, 天津.
 Nettles, C.G. and Susan, F. (1993) Biochemical and genetic characteristics of bacteriocin of food-associated lactic acid bacteria. Food Protection, 50, 338-356．
 张卓然 (2004) 临床微生物学和微生物测试 (第三版). 人民卫生出版社, 北京.
 赵虎 (2005) 厌氧菌和微需氧菌感染与实验诊断. 上海科学技术出版杜, 上海.
 Ausubel, F.M., Kingston, R.E. and Seidman, J.G. (2005) Short protocols in molecular biology. 4th edition, Science Press, Beijing.
 Rajaram, G., Manivasagan, P. and Thilagavathi, B. (2010) Purification and characterization of a bacteriocin produced by Lactobacillus lactis isolated from marine environment. Advance Journal of Food Science and Technology, 2, 138- 144.
 Li, Y.L., Zhou, Z.J., Han, Y., Wang, P., Ding, C.W. and Bai, H. (2008) Isolation and purification of the antibacterial material produced by Pediococcus acidilactici. Food Science, 29, 271-274.
 Yang, R., Johnson, M.C. and Ray, B. (1992) Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Applied and Enviromental Microbiology, 58, 3355-3359.
 Hancock, R.E. and Chapple, D.S. (1999) Peptide antibiocics. Antimicrob Agents Chemother, 43, 1317-1323.
 Carraturo, A., Raieta, K., Ottaviani, D. and Russo, G.L. (2006) Inhibition of Vibrio parahaemolyticus by a bacteri- ocin-like inhibitory substance (BLIS) produced by Vibrio mediterranei 1. Journal of Applied Microbiology, 101, 234- 241.
 张红星, 刘丽, 谢远红, 熊丽霞, 刘慧 (2010) 植物乳杆菌LH-09产细菌素的抑菌特性研究. 第一次细胞/分子生物学/生物物理学和生物工程国际会议论文集, 齐齐哈尔, 2010.12.25, 234-235.
 Martinez, F.A., Balciunas, E.M., Converti, A., Cotter, P.D. and de Souza Oliveira, R.P. (2013) Bacteriocin production by Bifidobacterium spp. A review. Biotechnology Advances, 31, 482-488.
 王占锋, 张萍, 魏萍 (2010) 肠道益生菌抗病毒作用及其机制研究进展. 中国微生态学杂志, 22, 184-185.