PtOEP氧气传感器实验研究
Experiment Research of PtOEP Oxygen Sensor

作者: 刘建斌 , 李 海 , 廖延彪 , 陈荣声 , 邓海东 , 曾应新 :华南农业大学电子工程学院,广州 广东;

关键词: 传感技术氧气传感器荧光猝灭PtOEPSensing Technology Oxygen Sensors Fluorescence Quenching PtOEP

摘要:
采用荧光物质PtOEP (platinum-octaethyl-porphyrin)作为指示剂,基于荧光猝灭原理设计了一种氧气传感器。设计思路为:首先,使用信号发生器对LED激发光源进行方波调制,然后,该光源照射在具有荧光指示剂的高分子材料PMMA(Polymethyl Methacrylate)上产生激发荧光,进而荧光强度也呈现方波变化,然后用气体混合器对氧气浓度与荧光强度的关系进行定标。该传感器具有检测精度高,较好的稳定性和重复性,并具有较快的响应速度。该传感器可用于测量未知环境(比如动物细胞培养环境)的氧气浓度。

Abstract: An oxygen sensor is designed using a fluorescent substance PtOEP (platinum-octaethyl-porphyrin) as an indicator, based on the principle of fluorescence quenching. Design idea is that: first, square wave modulation for LED excitation light source is made; second, the light irradiates in PMMA (Polymethyl Methacrylate) with fluorescent indicator, which produces fluorescence excitation and then fluorescence intensity also shows square wave change; Last the relationship between the oxygen concentration and fluorescence intensity is calibrated using gas mixer. The sensor has high precision, good stability and repeatability, and has faster response speed. The sensor can be used to measure the unknown environment (such as animal cell culture environment) of oxygen con-centration.

文章引用: 刘建斌 , 李 海 , 廖延彪 , 陈荣声 , 邓海东 , 曾应新 (2015) PtOEP氧气传感器实验研究。 光电子, 5, 1-5. doi: 10.12677/OE.2015.51001

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