Xuebijing Regulate the Hypothalamic-Pituitary-Adrenal Axis in Septic Rats
Abstract: Objective: To investigate the level changes of corticotropin-releasing hormone (CRH), adren- ocorticotropic hormone (ACTH) and cortisol (CORT) level in plasma in septic rats, without and with the use of Xuebijing injection. The main purpose is to reveal whether Xuebijing can affect the hypothalamus-pituitary-adrenal (HPA) axis in septic rats. Methods: Forty male Sprague- Dawley rats were randomly divided into 4 groups: control group, sham group, saline group (NS group), Xuebijing for treatment group. The septic rat models were challenged by CLP. We observe the clinical manifestation of animal after CLP. We use the radio-immunity method to detect the levels of CRH, ACTH and CORT in plasma. Results: Compared with the Sepsis group, the level of CRH, ACTH and CORT in XBJ group was significantly decreased (P < 0.05), but both groups were significantly higher than that in sham group. And the clinical manifestations in the rats of XBJ group were better. There was no significant difference between Normal control and Sham group (P > 0.05). Conclusion: In septic rats, the levels of the CRH, ACTH and CORT in plasma were increased, which indicate that HPA axis was more active. Xuebijing can decrease the levels of CRH, ACTH and CORT in plasma, downregulate overactivation of the HPA axis, improves the clinical manifestation.
文章引用: 胡艳玲 , 李宏山 , 刘淑正 (2015) 血必净对脓毒症早期大鼠下丘脑–垂体–肾上腺轴的调节。 临床医学进展， 5， 7-11. doi: 10.12677/ACM.2015.51002
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