黄单胞菌中c-di-GMP二鸟苷酸环化酶和磷酸二酯酶的生物信息学分析
Bioinformatics Analysis of Diguanylate Cyclases and c-di-GMP-Specific Phosphodiesterases from Xanthomonas Species

作者: 邹 霞 , 黄良博 , 何 进 :华中农业大学生命科学技术学院,农业微生物学国家重点实验室,湖北 武汉;

关键词: 黄单胞菌第二信使信号分子c-di-GMP二鸟苷酸环化酶磷酸二酯酶生物信息学Xanthomonas Second Messenger Molecule c-di-GMP Diguanylate Cyclase Phosphodiesterase Bioinformatics

摘要:
黄单胞菌是一类重要的作物病原菌,其信号转导机制及其与寄主植物的相互作用一直是微生物学、植物病理学及植物学工作者研究的热点问题。c-di-GMP是一种广泛存在于细菌中的第二信使分子,参与细菌的粘附、胞外多糖的合成、生物膜形成、运动性及毒力等许多细菌生理过程的调节。c-di-GMP主要由含有GG(D/E)EF结构域的二鸟苷酸环化酶合成,由含有EAL或HD-GYP结构域的磷酸二酯酶降解,其在胞内的浓度受到二鸟苷酸环化酶与c-di-GMP特异性磷酸二酯酶的协同调节。本文对黄单胞菌属中十五株具有完整基因组序列的菌株中含有这三类结构域的蛋白进行了包括分布规律、进化树、COG及GO分析在内的生物信息学分析,揭示了GG(D/E)EF、EAL与HD-GYP结构域蛋白在黄单胞菌属中的进化差异及其与侵染对象的关联性;研究结果也表明这些结构域蛋白主要为信号转导蛋白,参与基因转录和细胞运动。这些结果为构建c-di-GMP介导的调控网络和寻找新的控制黄单胞菌的药物靶标奠定了良好的基础。

Abstract: The genus Xanthomonas currently comprises 27 species that collectively cause serious diseases in a wide variety of economically important crops and horticultural plants. The bacterial signal transduction mechanism and the bacterium-host plant interaction have been hot issues for micro-biologists, plant pathologists and botanists. c-di-GMP is an ubiquitous bacterial second messenger. It participates in the regulation of many bacterial physiological processes, such as adhesion, EPS synthesis, biofilm formation, motility and virulence, etc. The c-di-GMP turnover is controlled by two functionally opposite enzymes: diguanylate cyclase containing GG(D/E)EF domain and c-di- GMP-specific phosphodiesterase containing EAL or HD-GYP domain. In this study, we performed bioinformatics analysis on the proteins containing GG(D/E)EF, EAL and HD-GYP domians from 15 Xanthomonas strains with complete genome sequences. The distribution and phylogenetic tree analysis revealed that the evolutionary differences of these proteins were related to their hosts; COG and GO analysis demonstrated that these functional proteins were mainly signal transduction related proteins, involved in gene transcription and bacterial motility. These results established solid foundation for the construction of the regulation network mediated by c-di-GMP and for the discovery of new drug targets for Xanthomonas control.

文章引用: 邹 霞 , 黄良博 , 何 进 (2014) 黄单胞菌中c-di-GMP二鸟苷酸环化酶和磷酸二酯酶的生物信息学分析。 计算生物学, 4, 68-82. doi: 10.12677/HJCB.2014.44008

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