Effect of Ethanol Extracts from Hericium erinaceum Solid-State Fermented Wheat on Nerve Cells Growth

作者: 何纯谊 , 陈淑德 , 吕宛儒 , 王桢翔 , 张永钟 :国立宜兰大学,食品科学系,台湾; 郑永祥 , 赖裕顺 :国立宜兰大学,生物技术和动物科技系,台湾;

关键词: 猴头菌固态发酵神经生长因子轴突Hericium erinaceus Solid-State Fermentation Nerve Growth Factor (Ngf) Neurite

神经生长因子(nerve growth factor, NGF)是神经营养因子之一,为神经细胞生长的重要因素,可防止神经退化。猴头菇(Hericium erinaceum)为一种重要的药膳兼用真菌,它含有猴头素和猴头酮等生理活性成分,可促进NGF的生成,增加神经轴突的生长。本研究通过猴头菌小麦固态发酵28天的乙醇萃取物与大鼠肾上腺髓质嗜铬细胞株(rat pheochromocytoma cell line, PC12)共培养,观察其生长情形及检测NGF含量。结果显示浓度为1~100 μg/mL的猴头菌小麦固态发酵萃取物(HEE)对PC12细胞无明显的细胞毒性作用。同时发现,添加10 μg/mL猴头菌固态发酵小麦的萃取物可显著促使PC12细胞产生轴突及分泌NGF。

Abstract: Nerve growth factor (NGF) is one of neurotrophic factors, which is responsible for neuronal growth and prevents from neurodegeneration. Hericium erinaceums (H. erinaceus) is an important medi-cinal and edible mushrooms, and it has bioactive components such as erinacines and hericenones, which can induce nerve growth factor (NGF) production, and increase the neurite outgrowth. This study was to observe the growth of PC 12 cell and analyze NGF contents by producing 28-days H. erinaceus solid-state fermented wheat and feeding the rat pheocromocytoma PC12 cell with the ethanol extracts. The concentration 1 - 100 μg/mL ethanol extracts (HEE) from H. erinaceum sol-id-state fermented wheat product did not significantly cause cytotoxicity of cultured PC12 cells. The NGF contents and neurite development of PC12 cells were significantly increased by adding 10 μg/mL 28-days HEE to medium.

文章引用: 何纯谊 , 陈淑德 , 吕宛儒 , 王桢翔 , 张永钟 , 郑永祥 , 赖裕顺 (2015) 猴头菌固态发酵小麦的萃取物对神经细胞生长的影响。 食品与营养科学, 4, 1-10. doi: 10.12677/HJFNS.2015.41001


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