Orthotropic Transplantation to Establish H22 Hepatocellular Carcinoma Model in Mice
Abstract: Objective: To establish a liver orthotropic transplantation tumor model with H22 cells, for the further development of the related experimental study. Methods: A certain number of H22 cells were directly injected in the left lobe of the liver in C57BL/6 mice, establishing a transplanted tu-mor model of hepatocellular carcinoma in situ. Results: After 7 days of surgery, the liver surface appeared irregular size nodules, nodules increased with the prolongation of time increases, the late abdominal organs appeared invasion, adhesion, ascites was increased. Histopathological ex-amination of liver tumor tissues and adjacent tissues of cancer showed that liver orthotropic transplantation tumor formation rate was 100%, no spontaneous regression. Conclusions: Ortho-tropic injection of H22 cell established orthotropic transplantation tumor formation in the liver, and the tumor formation time was relatively short, tumor formation processes associated with organ metastasis and ascites. This model was reasonable to simulate the pathological and physiological body of liver cancer in human, and could satisfy the requirement of research on liver cancer.
文章引用: 韩 琛 , 王恒孝 , 王朝霞 (2015) 原位移植法建立肝脏H22肝癌细胞移植瘤小鼠模型。 世界肿瘤研究， 5， 15-20. doi: 10.12677/WJCR.2015.51003
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