基因组定向编辑技术——CRISPR/Cas9的研究进展
Advances in Genome Directional Editing Technologies of CRISPR/Cas9

作者: 姚 瑶 , 钱雪艳 , 郭东全 :吉林省农业科学院农业生物技术研究所,长春;

关键词: CRISPR/Cas系统Cas9蛋白基因定点修饰CRISPR/Cas System Cas9 Protein Targeted Genome Modification

摘要:
CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats/CRISPR-associated)是广泛存在于细菌及古生菌中的,由细菌体长期进化而形成的,能够降解入侵病毒或噬菌体DNA的适应性免疫系统。由于CRISPR/Cas的Ⅱ型系统比较简单,因此成为目前应用最为广泛的方法。本文主要介绍了CRISPR/Cas系统的基本结构、作用原理、技术特点、研究进展以及该技术可能的应用前景等方面内容。

Abstract: CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated) refers to an adaptive immune system that is gained from the long-term evolution of the organism which is widespread in bacteria and archaea. The system is able to degrade the invading virus or phage DNA. TypeⅡ of CRISPR/Cas system has become the most popular method due to its simplicity. This paper summarizes the basic structure, principle, technical characteristics and the progress of CRISPR/Cas, as well as the application prospect of the technology.

文章引用: 姚 瑶 , 钱雪艳 , 郭东全 (2014) 基因组定向编辑技术——CRISPR/Cas9的研究进展。 农业科学, 4, 142-150. doi: 10.12677/HJAS.2014.46022

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