Dithiothreitol Inhibits the Amyloid Fibril Formation of β-Lactoglobulin
Abstract: Amyloid fibrils are associated with Alzheimer’s disease, Parkinson’s disease, and non-neuropathic systemic amyloidosis. The effect of DTT on the amyloid formation of β-lactoglobulin has been ex-amined by thioflavin T binding, Congo red binding and transmission electron microscopy. The re-sults showed that the formation of the amyloid fibril took place when β-lactoglobulin was incubated in the presence of 5M urea and at neural pH. However, the formation of amyloid fibrils was inhibited when DTT was added into the solutions and the inhibitory effect is dependent on the concentrations of DTT. These results demonstrate that the disulfide bonds play important roles in the formation of amyloid fibrils.
文章引用: 马保亮 , 魏良淑 , 卢礼萍 , 吴 芳 (2014) 二硫苏糖醇抑制乳球蛋白淀粉样纤维的形成。 生物物理学， 2， 39-44. doi: 10.12677/BIPHY.2014.24005
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