Pyrosequencing Analysis Reveals Abundance and Community Composition of Denitrifying Bacteria in Xilin River Sludge
Abstract: Nitrogen loss caused by denitrifying bacteria is an important link in the global carbon-nitrogen cycles, whose community compositions and abundance may have a role in regulating emissions and forms of gaseous nitrogen. Based on 454 pyrosequencing of the 16S rRNA gene, as well as the reported denitrifying bacterial genera mentioned in many literatures, we detected community compositions and abundance of denitrifying bacterial communities in Xilin River sludge. The results showed that 5744 quality reads were grouped into 2263 OTUs at genus level (0.05 distance). After alignment with known sequences deposited in GenBank, the unclassified bacteria genera accounted for 57.49%. 204 classified bacterial genera covered 20 denitrifying bacteria genera, including 1.40% of Flavobacterium, 1.23% of Hydrogenophaga, 0.19% of Bacillus and Thauera, 0.17% of Thiobacillus, 0.15% of Hyphomicrobium, etc. Among them, Flavobacterium and Hydrogenophaga were the most dominant denitrifying bacterial genera in Xilin River bottom sludge, accounting for 69.03% of the total denitrifying bacterial genera. Correlation analysis showed that Flavobacterium preferred to attach to coarse sand grains in relatively high pH conditions, promoting the emission of CH4 and inhibiting the production of N2O and CO2. In opposition, Hydrogenophaga showed special preference to fine silt grains in rich nutrition conditions, promoting the emission of N2O and CO2 and inhibiting the production of CH4.
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