难治性免疫性血小板减少症患者调节性T细胞检测及其意义
Detection and Significance of Regulatory T Cells in Patients with Refractory Immune Thrombocytopenia

作者: 赖宗浪 , 李冬云 , 雷 勇 , 褚雨霆 , 陈信义 , 许亚梅 :北京中医药大学东直门医院血液肿瘤科,北京;

关键词: 难治性免疫性血小板减少症调节性T细胞中医Refractory Immune Thrombocytopenia Regulatory T Cells Traditional Chinese Medicine

摘要:
目的:探索难治性免疫性血小板减少症患者外周血中调节性T细胞数量的变化,明确二者之间是否存在相关性。方法:采用流式细胞仪分别检测21例健康人,21例难治性ITP患者CD4+CD25+细胞和CD4+CD25+Foxp3+细胞的表达水平,将两组计量资料进行统计学分析。结果:难治性ITP患者中外周血CD4+CD25+细胞比例(4.52 ± 2.95)%、CD4+CD25+Foxp3+占CD4+细胞比例(2.30 ± 0.72)%,与正常对照组相比,均明显下降,差异具有统计学意义(P < 0.001, 0.05),与血小板下降数量无相关性(P > 0.05)。结论:难治性ITP患者体内调节性T细胞数量下降,考虑体内存在自身反应亢进,可破坏血小板,但两者无相关性,这可能也是导致ITP难治的原因之一。

Abstract: Objective: To explore the expression of regulatory T cells in peripheral blood in patients with re-fractory immune thrombocytopenia (ITP) and the association between them. Methods: 21 patients with refractory ITP and 21 health controls were studied. The expressions of CD4+CD25+ cells and CD4+CD25+Foxp3+ cells were detected by flow cytometry. Statistical analysis was carried out with regard to data in two groups. Results: The ratios of CD4+CD25+ cells and CD4+CD25+Foxp3+ cells/ CD4+ cells in patients with refractory ITP decreased significantly compared with those in the con-trol group ((4.52 ± 2.95)% vs (6.33 ± 2.93)%, P < 0.001; (2.30 ± 0.72)% vs (4.17 ± 1.23)%, P < 0.05). There was no correlation between platelet counts and Treg cells. Conclusions: Detection of Treg cells in patients with refractory ITP can induce the hyper auto-reactive activation and more damages of platelets, but they don’t have relations, which may be one reason of refractory ITP.

文章引用: 赖宗浪 , 李冬云 , 雷 勇 , 褚雨霆 , 陈信义 , 许亚梅 (2014) 难治性免疫性血小板减少症患者调节性T细胞检测及其意义。 临床医学进展, 4, 42-46. doi: 10.12677/ACM.2014.43008

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