Study on Microscopy Techniques for Identifying Lignin Distribution in the Stem of Sorghum
Abstract: Distribution of lignin in the stem of sorghum [Sorghum bicolor (L.) Moench] was examined by the visible light microscopy coupled with the Wiesner and Mäule color reactions and by autofluores-cence microscopy to provide effective and reliable methods for identification of lignin distribution in gramineous plants. Transverse sections stained with Wiesner reaction and safranin both showed that epidermic cells, mechanical tissue, vascular bundle sheaths and vascular bundles were ligni-fied. The middle lamella and cell corner appeared to contain more lignin than the secondary wall. Fluorescence microphotographs of sections stained with safranin and unstained demonstrated not only the tissues above contained lignin, but parenchyma cells showed bright autofluorescence. Mäule reagent staining showed that lignin in secondary walls of vascular bundle sheaths was rich in syringyl lignin, whereas middle lamellae and cell corner of vascular bundle sheaths and xylem cells lacked syringyl. The present study suggested that Wiesner reaction and safranin staining may be effectively applied in determining the distribution of lignin in cell walls of sorghum. Mäule reaction provides a rapid and sensitive estimate of syringyl lignin distribution. Autofluorescence examination under blue light excitation was sensitive and effective technique for total lignin dis-tribution in cell walls of gramineous plants.
文章引用: 邓建华 , 管梦灵 , 刘美芹 (2014) 高粱茎杆中木质素分布的显微技术研究。 植物学研究， 3， 188-194. doi: 10.12677/BR.2014.35024
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