气相色谱–质谱法测定保健品中厚朴酚与和厚朴酚含量
Determination of Magnolol and Honokiol Content in Health Products by Gas Chromatography Mass Spectrometry

作者: 袁 君 , 赵红峰 :普研标准技术服务有限公司,上海;

关键词: 厚朴酚和厚朴酚保健品Magnolol Honokiol Health Products

摘要:
利用超声处理,提取保健品中厚朴酚与和厚朴酚,最终确定氦气为流动相,色谱柱为DB-5(30 m × 0.25 mm × 0.25 μm),流速1.0 ml/min,程序升温条件为70℃保持2 min,以5℃/min的速率升温至230℃,保持20 min,进样方式为分流进样,分流比为50:1,分离效果良好,重复性好,回收率高,符合定性定量分析的要求。乙醇超声时间为30 min的条件下, 保健品中厚朴酚与和厚朴酚提取率较高。

Abstract: By ultrasound treatment, magnolol and honokiol in extraction of health products, ultimately de-termine the helium as the mobile phase. The column was DB-5 (30 m × 0.25 mm × 0.25 μm); flow rate was 1.0 ml/min; programmed temperature was 70˚C for 2 min; the temperature went up to 230˚C for 20 min with the rate of heating at 5˚C/min. Split injection was applied for sampling, where the split ratio is 50:1. This method had good separation effect, good repeatability, high recovery rate, which was consistent with the requirements of qualitative and quantitative analysis. The highest extraction rate of magnolol and honokiol in health products was obtained when the ethanol ultrasonic time is 30 min.

文章引用: 袁 君 , 赵红峰 (2014) 气相色谱–质谱法测定保健品中厚朴酚与和厚朴酚含量。 食品与营养科学, 3, 49-55. doi: 10.12677/HJFNS.2014.33009

参考文献

[1] 王大力, 孙艳涛, 杜长青 (2008) HPLC-MS厚朴有效成分研究. 延边大学学报(自然科学版), 4, 276-278.

[2] 任坚忍, 余凡, 张诗蓓, 等 (2011) 厚朴活性成分研究进展. 现代农业科技, 21, 127-128.

[3] 保志娟, 杨雪琼, 丁中涛, 等 (2004) 紫外分光光度法同时测定厚朴酚与和厚朴酚的含量及活性研究. 天然产物研究与开发, 5, 435-438.

[4] 杨红, 兵石磊, 詹亚华, 等 (2007) 湖北恩施州产厚朴的挥发油分析. 中国中药杂志, 1, 42-44.

分享
Top