Ultrastructural Observation and
Neurotransmitter Analysis of Tissue Regeneration after Amputation of Zebrafish Lower Jaw
Abstract: Zebrafish is an ideal model for studying organ regeneration, particularly in complicated nerve tissue structures like lower jaw and caudal fin. In this study, we dynamically observe ultrastructural changes of wound tissues and analyze four neurotransmitter contents during the early stage of lower jaw regeneration after removal of one third of mandible and associate soft tissues. Two hours post-amputation (2 hpa), contraction occurs around the edges of the wound causing the wound size to become smaller. In comparison with non-amputation, the level of dopamine, γ-Aminobutyric acid (GABA), glutamic acid and glycine increase in the wound tissues. The content of four neurotransmitters reaches to the peak when epidermis reconstitution is complete at 2 dpa. At 5 dpa, the missing structure is largely recovered while the 4 neurotransmitters retain the high level or slightly reduce. These results suggest that neurotransmitters play important roles in introducing lower jaw regeneration.
文章引用: 吴 昊 , 李 利 , 李先瑞 , 王箕雨 , 陈丹群 , 严继舟 (2014) 斑马鱼下颌损伤后的再生组织的超微结构观察及神经递质分析。 农业科学， 4， 73-80. doi: 10.12677/HJAS.2014.44012
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