厦门市售甲鱼蛋携带病原菌情况初步调查
Primary Investigation of Pathogenic Bacteria from Sold Terrapin Eggs in Xiamen

作者: 吴玉娟 :集美大学生物工程学院,厦门;厦门出入境检验检疫局,厦门; 孔繁德 , 徐淑菲 , 王仍瑞 :厦门出入境检验检疫局,厦门;

关键词: 甲鱼蛋MALDI-TOF-MS病原菌Terrapin Egg MALDI-TOF-MS Pathogen

摘要:

目的:了解厦门市售甲鱼蛋携带病原菌情况。方法:按照病原菌的检测标准进行分离培养,获得纯培养物后,用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)结合相应的诊断血清及PCR方法进行鉴定。结果:自7批厦门市售甲鱼蛋中分离到了16个属26种共76株细菌,甲鱼蛋带菌率为100%,多重带菌情况严重。结论:该次甲鱼蛋的检验结果表明,甲鱼蛋所携带病原菌种类广,大部分为多重带菌,部分携带致病菌或条件致病菌。因此必须做好甲鱼蛋的检疫工作,以确保人们身体健康和养殖业的健康持续发展。

Abstract: Objective: To explore the situation of pathogens of Xiamenlocal terrapin eggs. Methods: The pathogens were isolated with standard methods. The pure cultures isolated from terrapin eggs were further specified by MALDI-TOF-MS, serological test and PCR methods. Results: 100% of 7 batches of Xiamen local terrapin eggs were contaminated with pathogenic bacteria according to MALDI- TOF-MS. 76 strains of isolated pathogenic bacteria fell into 16 genera and 26 species. All of the terrapin eggs carried five or more pathogenic bacteria. Conclusion: The results suggest that high- contamination rate, broad genera, coexistence of multiple species and isolation of pathogenic bacterium are very common phenomena. It’s very necessary to enforce surveillance of pathogenic bacteria in terrapin eggs.

文章引用: 吴玉娟 , 孔繁德 , 徐淑菲 , 王仍瑞 (2014) 厦门市售甲鱼蛋携带病原菌情况初步调查。 微生物前沿, 3, 36-43. doi: 10.12677/AMB.2014.32005

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