Study on Extraction, Isolation, Purification and Physical Chemistry Properties of the Glycoprotein from Thais clavigera Kuster
Abstract: Purpose: This paper studied on extraction, isolation and purification as well as physical chemistry properties of the Glycoprotein from Thais clavigera Kuster. Method: The crude extract was then fractionated with 3 concentrations of ammonium sulfate solution. LGP-1, LGP-2, LGP-3, LGP-4 were prepared from the crude through DEAE-cellulose. LGP-I, LGP-II, LGP-III, LGP-IV, LGP-V, LGP-VI, LGP-VII were prepared through Sephadex G-100 chromatography. Result: The results showed that three glycoprotein components were carried out from 40% grade ammonium sulphate precipitation, named LGP-I, LGP-II, LGP-III. Three peaks appeared for 60%s, named LGP-IV, LGP-V and LGP-VI. The sugar were 17.86%, 20.00%, 13.14%, 21.57%, and the contents of protein were 56.72%, 62.58%, 71.86%, 60.86%. The IR indicated glycoprotein groups contained −OH, −COO−, −NH, −C−O−C characteristics.
文章引用: 蔡和君 , 葛飞飞 , 俞 斌 , 赵 盼 , 曲有乐 (2014) 辣螺糖蛋白的提取、分离纯化及理化性质分析。 海洋科学前沿， 1， 1-8. doi: 10.12677/ams.2014.11001
 Kamjya, H. and Muramoto, K. (1988) Studies on bioactive marine metabolites—Characterization of the antibacterial and antineoplastic glycoprotein in a sea hare Aplysia juliana. Nippon Suisan Gakkaishi, 54, 737-777.
 Kisugi, J., Ohye, H. and Kamjya, H. (1989) Biopolymers from marine invertebrates. X. mode of action of an antibacterial glycoprotein, aplysianin E, from eggs of a sea hare, Aplysia kurodai. Chemical and Pharmaceutical Bulletin, 37, 3050-3053.
 Takuma, S., Noriko, A. and Hiroyoki, U. (1985) Antitumor activity of aqueous extracts of marine animals. Journal of Pharmacobio-Dynamics, 8, 996-974.
 Tapieroh, H. and Tew, K.D. (1996) Increaeed glutathione expression in cells induced by Crassostera gigas extract (JCOE). Biomedicine & Pharmacotherapy, 50, 149-153.
 Yoshikawat, T., Naito, Y., Masui, K., et al. (1997) Free radical-scavenging activity of Crassostera gigas extract (JCOE). Biomedicine & Pharmacotherapy, 51, 328-332.
 顾谦群, 方玉春, 辛现良, 等 (2001) 栉孔扇贝糖蛋白的肿瘤抑制活性和对免疫功能的影响. 营养学报, 23, 200-202.
 傅余强, 顾谦群, 刘睿, 等 (2002) 管角螺肌肉中性糖蛋白的化学组成及抗肿瘤活性研究. 中国海洋药物, 6, 20-24.
 Matsuia, M., Kakuta M. and Misaki, A. (1996) Fine Structural features of oyster glycogen: Mode of multiple branching. Carbonhydrate Polymers, 31, 227-235.
 孙册, 莫汉庆 (1998) 糖蛋白与蛋白聚糖结构、功能和代谢. 科学出版社, 北京.
 武金霞, 赵晓瑜 (1993) 糖蛋白的结构、功能及分析方法. 中国生化药物杂志, 1, 31-34.