Preparation and Glucose Oxidase Adsorption Performance of Carbonaceous Mesocellular Foams
Abstract: By using fluorine-modified and original mesostructured cellular foam silica as templates, furfuryl alcohol as carbon precursors, carbonaceous mesocellular foams with different pore sizes were prepared, and then the grain sizes and pore structures of the samples were characterized by scanning electron microscope (SEM), Transmission Electron Microscopy (TEM) and nitrogen adsorption. It was found that fluorine-modified mesostructured cellular foams possessed the smaller particle sizes and specific surface area, but the larger windows and cell diameters and the larger pore volumes. All prepared samples were used as the carriers for immobilization of glucose oxidase. The loading amounts on carbonaceous mesocellular foams were much higher than on corresponding silicon templates, indicating that the immobilization process might be affected strongly by the electrostatic repulsion between the glucose oxidase molecules and carrier surface. Furthermore, carbonaceous mesocellular foam with smaller particle size, larger window size and pore volume showed the highest glucose oxidase loading amounts. It might be served as the prospective enzyme carrier material.
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