少花龙葵花药发育中多糖和脂滴的组织化学研究
Histochemical Observation of Polysaccharides and Lipids on the Developing Anthers of Solanum photeinocarpum

作者: 郭笑娟 , 郭娇娇 , 宋铁蛋 , 田惠桥 :厦门大学生命科学学院,厦门;

关键词: 少花龙葵花药发育多糖脂滴Solanum photeinocarpum Anther Development Polysaccharides Lipid

摘要:
对少花龙葵花药发育中淀粉粒和脂滴的分布和转化过程进行组织化学研究。结果显示:在造细胞时期幼小花药中没有营养物质积累;在小孢子母细胞时期,花药表皮和药室内壁细胞中出现淀粉粒,同时在绒毡层细胞中出现了较多的脂滴;在四分体时期,中层细胞中出现淀粉粒,而绒毡层细胞中的脂滴减少;到小孢子早期,花药表皮细胞中除淀粉粒外还出现了脂滴;但到小孢子晚期,药壁细胞中的淀粉和脂滴都明显减少;在二胞花粉早期,花粉开始积累淀粉粒,药壁细胞中的淀粉和脂滴几乎消失;在即将开花的成熟花粉中积累了大量淀粉粒和少量脂滴作为其存储物。

Abstract:
The distribution of polysaccharides and lipid drops in developing anther of Solanum photeinocarpum was studied. At the sporogenous cell stage, neither starches nor lipids were in anther wall cells. At the stage of microspore mother cells, some starches appeared in epidemic cells and endothecium cells and many lipids appeared in tapetal cells. At the tetrad stage, some polysaccharides appeared in middle layer cells. After microspores were released from tetrads, both starches and lipids appeared in epidemic cells, but both materials decreased at the late microspore stage. After the microspore division, early bicellular pollen began to accumulate polysaccharides, and the starches and lipids disappeared in anther wall cells. With the pollen development, many starches and some lipids were accumulated in the vegetative cell of the nearly mature pollen.

文章引用: 郭笑娟 , 郭娇娇 , 宋铁蛋 , 田惠桥 (2014) 少花龙葵花药发育中多糖和脂滴的组织化学研究。 植物学研究, 3, 27-31. doi: 10.12677/BR.2014.31006

参考文献

[1] 胡适宜 (2005) 被子植物生殖生物学. 高等教育出版社, 北京, 29-92.

[2] Rudramuniyappa, C.K. and Annigeri B.G. (1985) Histochemical observations on the sporogenous tissue and tapetum in the anther of Euphorbia. Cytologia, 50, 39-48.

[3] Tiwari, S.C. and Gunning, E.S. (1986) An ultrastructural cytochemical and immunofluorescence study of postmeiotic development of plasmodial tapetum in Tradescantia virginiana L. and its relevance to the pathway of sporopollenin secretion. Protoplasma, 133, 100-144.

[4] Calzoni, G.L., Speranza, A., Caramiel, T.R., et al. (1990) Wall ultrastructure and biochemical features of the Juglaus regia L. and Juglans nigra L. Male gametophyte. Sex Plant Reprod, 3, 139-146.

[5] 全国草药汇编编写组 (1996) 全国中草药汇编(第二版). 人民卫生出版社, 北京, 265-266.

[6] 邓汝铭, 黄瑞松, 苏青等 (1999) 少花龙葵的生药研究. 广西中医药, 5, 44-46.

[7] 徐淑元, 孙怀志, 谭雪 (2002) 保健野生蔬菜——少花龙葵的栽培技术. 广西园艺, 4, 30-31.

[8] 李芸瑛, 黄丽华, 陈雄伟 (2006) 野生少花龙葵营养成分的分析. 中国农学通报, 2, 101-102.

[9] 赵则海 (2008) 发育时期对少花龙葵光合生理特性及代谢产物的影响. 生态环境, 1, 312-316.

[10] 胡适宜, 徐丽云 (1990) 显示环氧树脂厚切片中多糖、蛋白质和脂类的细胞化学方法. 植物学报, 11, 841-846.

[11] 谢潮添, 杨延红, 朱学艺等 (2004) 白菜细胞核雄性不育花药的细胞化学观察. 实验生物学报, 4, 295-302.

[12] 包晗, 宋玉燕, 马燕等 (2011) 水鬼蕉花药发育中的多糖和脂滴组织化学研究. 植物生理学报, 6, 565-569.

[13] 李东霄, 李淦, 冯素伟等 (2013) 温敏核不育小麦可育和不育花药的细胞化学观察. 作物学报, 5, 878-884.

[14] 马燕, 邓炜, 田惠桥 (2011) 芒果花药发育的细胞化学研究. 广西植物, 3, 338-341.

[15] 尹增芳, 樊汝汶 (1998) 中国鹅掌楸小孢子发生的细胞化学研究. 植物学通报, 3, 34-37.

[16] 徐青, 王仙琴, 田惠桥 (2006) 枸杞花药发育过程中脂滴和淀粉粒的分布特征. 分子细胞生物学报, 2, 103-110.

[17] 杨淑娟, 魏冬梅, 成诚等 (2013) 凤仙花花药发育中多糖和脂滴组织化学研究. 西北植物学报, 9, 1786-1791.

[18] Liu, R.S., Qiu, Y.L., Wei, D.M., et al. (2011) Distribution of starch and neutral lipids in the developing anthers of Ipomoea cairica. Annales Botanici Fennici, 48, 256-262.

[19] El-Ghazaly, E.G. and Jensen, W.A. (1987) Development of wheat (Triticum aestivum) pollen. II. Histochemical differentiation of wall and Ubisch bodies during development. American Journal of Botany, 74, 1396-1418.

[20] Pacini, E., Franchi, G.G. and Hesse, M. (1985) The tapetum: Its form, function and possible phylogeny in Embryophyta. Plant Systematics and Evolution, 149, 155-185.

[21] Pacini, E. and Franchi, G.G. (1991) Diversification and evolution of the tapetum. In: Blackmore, S. and Barnes, S.H., Eds., Pollen and Spores: Patterns of Diversification. Systematics Association, Clarendon, Oxford, 301-316.

[22] 魏冬梅, 菅明霞, 邓桦等 (2007) 洋葱花药发育过程中的细胞化学研究. 分子细胞生物学报, 6, 451-457.

[23] 袁亦楠, 朱德蔚, 连勇等 (2000) 番茄雄性不育突变体小孢子发育的细胞学研究. 华北农学报, 3, 61-65.

[24] 邱义兰, 刘珠丽, 李红等 (2008) 辣椒细胞质雄性不育花药败育及淀粉粒分布的细胞学观察. 分子细胞生物学报, 4, 283-293.

[25] 罗玉英, 李怀军, 刘玉乐等 (1998) 转基因雄性不育烟草花药绒毡层及花粉发育的特点. 遗传, 4, 51.

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