粒毛盘菌胞内黑色素的提取工艺、红外光谱及抗氧化活性
Extraction Technology, Infrared Spectra and Antioxidant Activity of Intracellular Melanin from Lachnum

作者: 卢 莹 , 郭赓艺 , 李 兰 , 叶 明 :合肥工业大学,生物与食品工程学院,合肥; 殷坤才 :马鞍山市安康菌业有限公司,马鞍山市;

关键词: 粒毛盘菌黑色素提取工艺得率结构特征抗氧化活性Lachnum Melanin Extraction Technology Yield Structural Characteristic Antioxidant Activity

摘要:
采用碱溶酸沉法、超声波辅助提取法以及酶解法提取粒毛盘菌YM404胞内黑色素(LIM404),并在单因素实验的基础上,运用响应面法优化获得超声波辅助法的最佳提取条件为:NaOH浓度1.5mol·L-1、超声功率82 W、提取温度75、提取时间33 min,在此条件下提取的黑色素(LIM404-b)得率最高,为12.50%,较碱溶酸沉法获得的黑色素(LIM404-a)、酶解法获得的黑色素(LIM404-c)得率分别提高了5.04%23.76%。红外光谱显示,LIM404-b的结构特征与LIM404-aLIM404-c的基本相似。LIM404-b浓度为500mg·L-1时,与1.4251mmol·mL-1 α-生育酚的总抗氧化能力相当,对·OHDPPH··的清除率分别为64.43%76.05%68.43%,表明LIM404-b具有较强的抗氧化活性。
Intracellular melanins from Lachnum YM404 (LIM404) were obtained by the methods of alkali solution and acid isolation precipitation, ultrasonic-assisted extraction, enzyme hydrolysis, respectively. Based on the single factor experiments, Box-Behnken experiment was designed to obtain the optimum ultrasonic-assisted extraction conditions of LIM404: NaOH 1.5 mol·L-1, ultrasonic power 82 W, ultrasonic temperature 75˚C and ultrasonic time 33 min. Under these conditions, the yield of melanin (LIM404-b) was 12.5%, and increased 5.04%, 12.15% than that by alkali solution and acid precipitation, enzyme hydrolysis, respectively. FT-IR results showed that the structural characteristics of LIM404-b were similar to that of LIM404-a, LIM404-c. When the LIM404-b concentration was 500 mg·L-1, its total antioxidant capacity was equivalent to α-tocopherol of 1.4251 mmol·mL-1, and the ·OH, DPPH·and · scavenging rates were 64.43%, 76.05%, 68.43%, respectively, suggesting that the antioxidant activity of LIM404-b was strong.

文章引用: 卢 莹 , 郭赓艺 , 李 兰 , 殷坤才 , 叶 明 (2013) 粒毛盘菌胞内黑色素的提取工艺、红外光谱及抗氧化活性。 微生物前沿, 2, 91-97. doi: 10.12677/AMB.2013.24017

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