稀有植物山东银莲花(Anemone shikokiana (Makino) Makino)的核型分析及其生境对染色体变异的影响
Karyotype Analysis and Influence of Habitats on Chromosomal Variation of Rare Plant Anemone shikokiana (Makino) Makino
Abstract: Anemone shikokiana (Makino) Makino, which has important research values, is a rare plant. The species has two different habitats, the shrubs in mountaintop and understorey of the conifer and broad-leaf mixed forest. Karyotype is very important for the other researches of a plant. The chromosome numbers and karyotype of A. shikokiana were firstly studied using a traditional squash method and Leven’s karyotype analysis standard. The results showed that the karyotype of metaphase chromosomes in somatic cells is as follows: the number of chromosomes is 14, karyotypic formula is 2n = 2X = 14 = 12m + 2t, the A.S.K% is 56.5%, and the relative length of chromosome is 2n = 2X = 14 = 12L + 2M1. For exploring the influences of habitats on chromosomal variation, the morphology of chromosomes was analysed by nested analysis. The results showed that chromosomal variation is not evident between populations in different habitats, but obvious within the same population. So we think that chromosomal variation of A. shikokiana presents in the small scope, and chromosomal variation in large scale may cancel each other out because of enough large scale. This study will provide the basis and reference for future work on A. shikokiana.
文章引用: 王 鸷 , 逄玉娟 , 刘传林 , 庄树宏 , 卞福花 (2013) 稀有植物山东银莲花(Anemone shikokiana (Makino) Makino)的核型分析及其生境对染色体变异的影响。 植物学研究， 2， 113-116. doi: 10.12677/BR.2013.25020
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