陆地棉、澳洲棉和绿顶棉三元杂种的细胞遗传学研究
Cytogenetic Study on Trispecific Hybrid from Gossypium hirsutum L., G. australe and G. capitis-viridis

作者: 李灵娇 , 吴玉香 :山西农业大学农学院; 陈 弟 :中国农业科学院棉花研究所;

关键词: 陆地棉澳洲棉绿顶棉三元杂种减数分裂G. hirsutum G. australe G. capitis-viridis Trispecific Hybrid Meiosis

摘要: 棉属有丰富的种质资源,野生棉具有栽培种所缺乏的许多优良性状,为了将野生澳洲棉和绿顶棉的优良性状导入陆地棉,以期改良栽培种陆地棉。本研究通过陆地棉和C组野生澳洲棉远缘杂交获得杂种F1,并对其进行染色体加倍,将加倍成的异源六倍体与B组野生绿顶棉杂交,产生陆地棉、澳洲棉、绿顶棉的三元杂种,并对该三元杂种花粉母细胞减数分裂行为进行观察。结果表明:三元杂种减数分裂异常,主要表现在:后期染色体不均等分离,使得末期形成大量的不正常多分孢子,其中以五分体居多,这些多分孢子进一步发育为大量败育花粉粒,导致该杂种高度不育。本研究不仅为该三元杂种不育提供了直接原因,也为进一步探讨有效的育性恢复方法及棉花新种质的创制提供了理论依据和中间材料。

Abstract:  There are rich germplasm resources in Gossypium and wild cotton species possess many valuable agronomic traits which cultivated cotton always lacks. In order to introgress the useful traits from wild species G. australe and G. capitis-viridis into cultivated species for improving upland cotton, tetraploid G. hirsutum was crossed directly with C-genome species G. australe as the pollen-providing parent, creating a triploid hybrid. Chromosome doubling of this triploid hybrid leads to an allohexaploid which was then crossed with B-genome wild species G. capitis-viridis, resulting in trispecific hybrid (G. hirsutum, G. australe and G. capitis-viridis). The characteristic of chromosome behavior during meiosis of trispecific hybrid F1 was further investigated in this study. Results indicated that the meiosis of trispecific hybrid was abnormal, which mainly reflected that the chromosomes were divided unequally during the anaphase II; and many abnormal multispores appeared in the telophase II, which finally developed into abortive pollen grains, directly leading to the sterility of trispecific hybrid F1. This study provides the main reason of sterility for this hybrid and supplies the theory basis and the effective method for restoring the fertility and further creating new cotton intermediate germplasms.

文章引用: 李灵娇 , 陈 弟 , 吴玉香 (2013) 陆地棉、澳洲棉和绿顶棉三元杂种的细胞遗传学研究。 农业科学, 3, 89-94. doi: 10.12677/HJAS.2013.34018

参考文献

[1] P. A. Fryxell. A revised taxonomic interpretation of Gossypium L. (Malvaceae). Pheedea, l992, 2(2): 108-165.

[2] 王坤波, 杜雄明, 宋国立. 棉花种质创新的现状与未来[J]. 植物遗传资源学报, 2004, 5: 23-28.

[3] Y. Q. Sun, S. M. Liu, Y. Wang, et al. An interspecific somatic hybrid between upland (G. hirsutum L.cv.ZDM-3) and wild diploid cotton (G. klotzschianum A.). Plant Cell and Organ Culture, 2011, 106(2): 425-433.

[4] 梁正兰. 棉花远缘杂交的遗传与育种[M]. 北京: 科学出版社, 1999: 1-2.

[5] 梁正兰, 孙传渭. 棉花远缘杂交[M]. 北京: 科学出版社, 1982: 32.

[6] S. S. Mehetre, M. Gomes and E. Susan. RAPD and Cytomor- phological Analyses of F1, F2 and Amphidiploid (A1) Gene- rations of Gossypium arboretum × Gossypium capitis-viridis. Cytologla, 2004, 69(4): 367-379.

[7] P. J. Samora, D. M. Stelly and R. J. Kohel. Localisation and mapping of the Le1 and gl2 loci of cotton (Gossypium hirsutum). Journal of Heredity, 1994, 85: 152-157.

[8] S. C. McMichael. Combined effects of glandless genes Gl2 and Gl3 on pigment glands in the cotton plant. Agronomy Journal, 1960, 52: 385-386.

[9] J. C. McCarty, P. A. Hedin and R. D. Stipanovic. Cotton Gos- sypium spp. plant gossypol contents of selected Gl2 and Gl3 alleles. Journal of Agricultural and Food Chemistry, 1996, 44: 613-616.

[10] H. Muramoto. Hexaploid cotton: Some plant and fiber properties. Crop Science, 1969, 9(1): 27.

[11] 梁理民, 刘有良, 王增信等. 陆地棉 × 斯特提棉种间杂交创造抗枯黄萎病新种质[J]. 西北农业学报, 2002, 11(4): 16-18.

[12] 庞朝友, 杜雄明, 马峙英. 具有野生棉外源基因的陆地棉特异种质创造与利用进展[J]. 棉花学报, 2005,17(3): 171-177.

[13] 李懋学, 张赞平. 作物染色体及其研究技术[M]. 北京: 中国农业出版社, 1996: 63-66.

[14] 邓衍明,叶晓青,佘建明等. 植物远缘杂交育种研究进展[J]. 华北农学报, 2011, 26: 52-55.

[15] 汤访评. 菊属与四个近缘属植物远缘杂交研究[D]. 南京: 南京农业大学, 2009.

[16] S. G. Ram, V. Thiruvengadam, S. H. Ramakrishnan, et al. Inves- tigation on pre-zygotic barriers in the interspecific crosses invol- ving Gossypium barbadense and four diploid wild species. Euphytica, 2007, 159: 241-248.

[17] 孟金陵. 植物生殖遗传学[M]. 北京: 科学出版社, 1997.

[18] 郭宝德, 黄穗兰, 冀丽霞. 抗蚜棉花新品种晋棉51号的选育及高产栽培技术[J]. 山西农业科学, 2011, 39(9): 933-935.

[19] Atanassov II, S. A. Atanassova, A. I. Dragoeva, et al. A new CMS source in Nicotiana developed via somatic cybridization between N. tabacum and N. Alata. Theoretical and Applied Genetics, 1998, 97: 982-985.

[20] X. S. Yu, B. J. Chu, R. E. Liu, et al. Characteristics of fertile somatic hybrids of G. hirsutum L. and G. trilobum generated via protoplast fusion. Theoretical and Applied Genetics, 2012, 125(7): 1503-1516.

[21] X. Cheng, S. M. Chen, F. D. Chen, et al. Interspecific hybrids between Dendranthema morifolium (Ramat.) Kitamura and D. nankingense (Nakai) Tzvel. Achieved using ovary rescue and their cold tolerance characteristics. Euphytica, 2010, 172: 101- 108.

[22] 孙敏红, 张蜀宁. 多倍体育种在园艺作物中的应用[J]. 江苏农业科学, 2004, 1: 68-72.

[23] A. F. Robinson. Reniform in U.S. cotton: When, where, why, and some remedies. Annual Review of Phytopathology, 2007, 45: 111-125.

[24] 中国农业科学院棉花研究所. 中国棉花遗传育种学[M]. 山东: 山东科学技术出版社, 2003: 270.

[25] M. S. Brown, M. Y. Menzel. Polygenomic hybrids in Gossypium L. Cytology of hexaploids, pentaploids and hexaploid combi- nations. Genetics, 1952, 37: 242-263.

[26] J. O. Beasley. Meiotic chromosome behavior in species,species hybrids, haploids and induced polyploids in Gossypium. Ge- netics, 1942, 27: 25-54.

[27] M. Vafaie-Tabar, S. Chandrashekran. Meiosis in a triploid hybrid of Gossypium: High frequency of secondary bipolar spindles at metaphase II. Journal of Genetics, 2007, 86: 45-49.

[28] H. Benbouza, J. M. Lacape, J. M. Jacquemin, et al. Introgression of the low-gossypol seed & high-gossypol plant trait in upland cotton: Analysis of [(Gossypium hirsutum × G. raimondii)² × G. sturtianum] trispecific hybrid and selected derivatives usi- ng mapped SSRs. Molecular Breeding, 2010, 25(2): 273-286.

[29] 张伯静, 李炳林, 祝水金等. 亚洲棉、比克氏棉和陆地棉异源四倍体的合成[J]. 棉花学报, 1993, 5(1): 15-20.

[30] 高燕会, 祝水金, 季道藩. 四种栽培棉合成的四元杂种Fl细胞遗传学研究[J]. 棉花学报, 2003, 15(5): 259-263.

[31] 王志龙, 姜茹琴, 何鉴星等. 棉属海岛棉×拟似棉F1不育性研究[J]. 遗传学报, 1997, 24(4): 368-372.

[32] 于卓. 几种小麦族禾草及其杂交后代农艺特性的研究[J]. 草业学报, 2003, 3(12): 83-89.

[33] 杨杰. 温度对亚种间杂种花粉育性的影响[J]. 中国水稻科学, 2003, 17(2): 145-148.

[34] 吴玉香, 陈崇乾, 高燕会等. 活体保存对栽培棉种间四元杂种育性和染色体构型的影响[J]. 棉花学报, 2007, 19(6): 461- 466.

[35] G. S. Newaskar, V. P. Chimote, S. Subhash, et al. Interspecific hybridization in Gossypium L.: Characterization of progenies with different ploidy-confirmed multigenomic backgrounds. Plant Breeding, 2013, 132: 211-216.

分享
Top