SiRNA-ID-1对人肝癌细胞HepG2裸鼠皮下移植瘤生长的影响
The Effects of RNA Interference Targeting ID-1 on the Growth of Hepatocellular Carcinoma Xenografts in Nude Mice

作者: 苏春康 * , 苏富丽 , 郑洪裕 , 张建丹 , 胡恩杰 :福建省永定县医院,龙岩; 叶加建 :福建省老年医院,福州; 吴泉明 :福建省临床检验中心,福州;

关键词: siRNAID-1肝癌细胞转染肿瘤抑制 RNA Interference ID-1 Hepatocellular Carcinoma Transfection Inhibition Tumor

摘要:

目的:观察以RNA干扰技术沉默ID-1表达对人肝癌细胞HepG2裸鼠皮下移植瘤生长的抑制作用,探讨抑制ID-1表达对肝癌可能存在的抑癌作用。方法:向BALB/C-nu系雄性裸鼠皮下注射HepG2细胞建立裸鼠荷瘤模型,成瘤后随机将裸鼠分为空白组、转染对照组和siRNA-ID-1处理组,在第1、4、7、10和13 d分别注射等量的生理盐水、control-siRNA和甲基化的siRNA-ID-1。每周测量肿瘤体积,绘制肿瘤生长曲线。于第28 d处死裸鼠,取肿瘤组织行常规HE染色。结果:siRNA-ID-1处理组裸鼠肿瘤体积较空白组和转染对照组的体积小(P < 0.05);转染后癌细胞凋亡增多,空白组及转染对照组瘤细胞排列密集,细胞界限不清,生长活跃,核大深染,有较多核分裂象。结论:以siRNA技术沉默肝癌移植瘤组织中的ID-1表达可抑制肿瘤的生长,ID-1具有促肿瘤生长作用。

Objective: To investigate the inhibition effects of RNA interference targeting ID-1(si-RNA-ID-1) on the growth of Hepatocellular Carcinoma(HCC) in nude mice and the potential mechanism of ID-1 inthe development of HCC. Methods: HCC xenografts were developed by injecting HepG2 cells into BALB/C-nu nude mice. The tumor- bearing mice were randomly divided into 3 groups, including blank control group, control-siRNA group and siRNA-ID- 1 group. Each group was treated by isovolumetric normal saline, control-siRNA or siRNA-ID-1 on day 1, 4, 7, 10 and 13, respectively. The growth curves of HCC xenografts were made by the tumor sizes, which were measured per week. The nude mice were executed on day 28. The tumor tissue was prepared for the HE staining. Results: The tumor sizes in siRNA-ID-1 group were significantly smaller than that in control groups (P < 0.05). The apoptotic cells observed in siRNA-ID-1 group were increased in HE slides. Conclusions: The growth of HCC xenografts could be inhibited by si-RNA-ID-1 injection. Both proliferation inhibition and apoptosis induction play important roles in this progress. The results implied that ID-1 might be a potential target for the therapeutic strategy of HCC.

文章引用: 苏春康 , 苏富丽 , 郑洪裕 , 张建丹 , 胡恩杰 , 叶加建 , 吴泉明 (2013) SiRNA-ID-1对人肝癌细胞HepG2裸鼠皮下移植瘤生长的影响。 世界肿瘤研究, 3, 38-43. doi: 10.12677/wjcr.2013.34007

参考文献

[1] 刘豫瑞, 方明霞, 曾达武. Id-1在肝细胞癌中的表达及其临床意义[J]. 福建医科大学学报, 2009, 43(1): 32-35.

[2] R. Ding, S. Han, Y. Lu, C. Guo, H. Xie, N. Zhang, Z. Song, L. Cai, J. Liu and K. Dou. Overexpressed Id-1 is associated with patient prognosis and HBx expression in hepatitis B virus-re- lated hepatocellular carcinoma. Cancer Biology & Therapy, 2010, 10(3): 105-107.

[3] Y. C. Wong, X. Wang and M. T. Ling. Id-1 expression and cell survival. Apoptosis, 2004, 9(3): 279-289.

[4] 吴育晶, 金娟, 胡姗姗, 王迪, 张玲玲, 孙妩弋, 魏伟. 儿茶素对人肝癌细胞HepG2的影响[J]. 中国药理学通报, 2010, 26(12): 1598-1602.

[5] Z. Dong, S. Liu, C. Zhou, T. Sumida, H. Hamakawa, Z. Chen, P. Liu and F. Wei. Overexpression of Id-1 is associated with tumor angiogenesis and poor clinical outcome in oral squamous cell carcinoma. Oral Oncology, 2010, 46(3): 154-157.

[6] S. Bertin, T. Mohsen-Kanson, P. Baqué, A. Gavelli, D. Momier, F. Anjuere, G. F. Carle and V. Pierrefite-Carle. Tumor microen- vironment modifications induced by soluble VEGF receptor ex- pression in a rat liver metastasis model. Cancer Letters, 2010, 298(2): 264-272.

[7] M. K. Maw, J. Fujimoto and T. Tamaya. Expression of the in- hibitor of DNA-binding (ID)-1 protein as an angiogenic media- tor in tumour advancement of uterine cervical cancers. British Journal of Cancer, 2008, 99(10): 1557-1563.

[8] R. Zhao, X. Q. Liu, X. P. Wu, Y. F. Liu, Z. Y. Zhang, G. Y. Yang, S. Guo, J. Niu, J. Y. Wang and K. S. Xu. Vascular endothelial growth factor (VEGF) enhances gastric carcinoma invasiveness via integrin alpha(v)beta6. Cancer Letters, 2010, 287(2): 150- 156.

分享
Top