烧伤后增生性瘢痕患者血清中TGF-β、VEGF的浓度检测及意义
Research of Change of Serum Concentration of Cytokines TGF-β and VEGF in Post-Burned Hypertrophic Scar Patients

作者: 沈 锐 , 张凤刚 , 杜永军 , 宋红梅 , 陈晓东 , 徐路生 :中山大学附属佛山医院整形外科; 张金明 :中山大学附属第二医院整形外科;

关键词: 增生性瘢痕烧伤后血清标记物TGF-βVEGFHypertrophic Scar Post-Burned Serum Marker TGF-β VEGF

摘要: 目的探讨烧伤后增生性瘢痕患者血清中TGF-βVEGF浓度变化及意义。方法选择20098~20118我科住院的烧伤后增生性瘢痕患者共74例,根据创面愈合后瘢痕增生时间长短分组,分为4组,选择12例正常人作为正常对照组,共5组。用酶联吸附法(ELISA)检测患者血清中TGF-βVEGF的浓度,5组之间统计比较分析。结果瘢痕患者血清中TGF-βVEGF含量与瘢痕增生时段关系密切,1~3月早期瘢痕组血清中TGF-βVEGF含量开始增加,随瘢痕的发展升高,4~6个月瘢痕组血清中TGF-βVEGF含量达到了高峰。随着瘢痕成熟,TGF-βVEGF浓度又逐渐下降。结论烧伤后增生性患者血清中TGF-βVEGF的含量可较好地反映增生性瘢痕代谢活跃程度,是增生性瘢痕增生活跃程度敏感的标记物。

Abstract: Objective: To explore the dose and its clinic significance of profibrotic cytokines of TGF-β and VEGF in the serum of hypertrophic scar patients at excessive stages. Methods: Serum samples of 74 inpatients were collected, who were admitted to our hospital between 2009, 8 and 2011, 8 and were suffering from post-burned hypertrophic scars at various stages. Serum samples of 12 cases of healthy persons were also collected as the control group. Hypertrophic scar groups were divided into 4 subgroups according to the phase of scars and detected the concentrations of TGF-β and VEGF in serum of the trials using the ELISA method. Results: The doses of TGF-β and VEGF began to increase in 1 to 3 months hypertrophic scar group and achieved summit concentration in 4 to 6 months scar group. The concentration of TGF-β and VEGF degreased gradually with the maturation of hypertrophic scars. Conclusions: High concentration of TGF-β and VEGF in the serum can reflect the level of metabolic activity in the tissue of hypertrophic scars. TGF-β and VEGF may be a promising marker in discerning the growth and development of hypertrophic scars.

文章引用: 沈 锐 , 张金明 , 张凤刚 , 杜永军 , 宋红梅 , 陈晓东 , 徐路生 (2013) 烧伤后增生性瘢痕患者血清中TGF-β、VEGF的浓度检测及意义。 外科, 2, 23-26. doi: 10.12677/HJS.2012.24006

参考文献

[1] G. B. Pitzer, K. G. Patel. Proper care of early wounds to optimize healing and prevent complication. Facial Plastic Surgery Clinics of North America, 2011, 19(3): 491-504.

[2] D. Honardoust, M. Varkey and Y. Marcoux. Reduced decorin, fibromodulin, and transforming growth factor-[beta]3 in deep dermis leads to hypertrophic scarring. Journal of Burn Care & Research, 2012, 33(2): 218-227.

[3] N. S. Mogili, V. R. Krishnaswamy, M. Jayaraman, et al. Altered angiogenic balance in keloids: A key to therapeutic intervention. Translational Research, 2012, 159(3): 182-189.

[4] D. S. Steinbrech, B. J. Mehrara, D. Chau, et al. Hypoxia upregu- lates VEGF production in keloid fibroblasts. Annals of Plastic Surgery, 1999, 42(5): 514-519; Discussion 519.

[5] T. A. Wilgus, A. M. Ferreira, T. M. Oberyszyn, et al. Regulation of scar formation by vascular endothelial growth factor. Labora- tory Investigation, 2008, 88(6): 579-590.

[6] J. Y. Yang, S. Y. Yang. Are auricular keloids and persisitent hy- pertrophic scars resectable? The role of intrascar excision. An- nals of Plastic Surgery, 2012, 69(6): 637-642.

[7] D. M. Perry, D. A. McGrouther and A. Bayat. Current tools for noninvasive objective assessment of skin scars. Plastic and Re- constructive Surgery, 2010, 126(3): 912-923.

[8] R. J. Matthew, G. S. Gautam, P. A. Joseph, et al. Novel therapies for scar reduction and regenerative healing of skin wounds. Trends in Biotechnology, 2008, 26(4): 173-180.

[9] 沈锐, 利天增. 组织缺氧与增生性瘢痕的关系[J]. 国际外科学杂志, 2006, 33(1): 70-73.

[10] J. A. Duncan, J. S. Bond, T. Mason, et al. Visual analogue scale and ranking: A suitable and sensitive method for assessing quality. Plastic and Re-constructive Surgery, 2006, 118(4): 909-918.

[11] 王炜. 整形外科学[M]. 杭州: 浙江科学技术出版社, 1999: 428.

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