食品中合成色素检测技术研究进展
Recent Advances in Detection Technologies of Synthetic Colorants in Foods

作者: 朱永红 :重庆市计量质量检测研究院食品质量监督检验研究中心,国家农副加工产品及调味品质量监督检验中心,;

关键词: 合成色素食用色素非食用色素检测食品Synthetic Colorants Edible Colorants Non-Edible Colorants Detection Foods

摘要:

食品中合成色素的使用十分常见,当前食品中滥用合成色素的现象较为普遍。开展食品中合成色素的检测对规范合成色素在食品中的使用具有重要意义。目前针对食品中合成色素的检测技术取得了许多进展,本文就食品中食用和非食用合成色素的种类、检测方法标准现状、高通量检测技术、样品前处理技术及色谱分离条件等进行了综述。

Abstract: The use of synthetic colorants in foods was common. The misuse of synthetic colorants in foods was also common. Detection of synthetic colorants in foods was necessary for regulating the use of synthetic colorants in foods. A lot of progresses have been achieved about the detection technologies of synthetic colorants in foods now. This paper reviewed the variety, detection method standard, high throughput detection method, sample pretreatment technologies, and chromatographic separation conditions etc. of edible and non-edible synthetic colorants in foods.

文章引用: 朱永红 (2013) 食品中合成色素检测技术研究进展。 食品与营养科学, 2, 29-37. doi: 10.12677/HJFNS.2013.23007

参考文献

[1] GB 2760-2011. 食品添加剂使用标准[S]. 2011.

[2] 全国打击违法添加非食用物质和滥用食品添加剂专项整治领导小组. 食品中可能违法添加的非食用物质和易滥用的食品添加剂品种名单(第四批)[Z], 2010年.

[3] 全国打击违法添加非食用物质和滥用食品添加剂专项整治领导小组. 食品中可能违法添加的非食用物质和易滥用的食品添加剂品种名单(第一批)[Z], 2008年.

[4] 邹志飞, 蒲民, 李建军等. 各国(地区)食用色素的使用现状与比对分析[J]. 中国食品卫生杂志, 2010, 22(2): 112-121.

[5] 葛宇. 食品中人工合成色素使用法规及检测标准进展[J]. 质量及标准化, 2011, (9): 31-35.

[6] 郑月明, 国伟, 聂雪梅等. 食品中违禁添加的非食用色素检测技术综述[J]. 中国农学通报, 2012, 280(9): 222-228.

[7] 王建伟, 梁炽琼. 食品中对位红等禁用染料检测方法的研究及最新进展[J]. 食品安全导刊, 2009, (7): 40-41.

[8] 卢土英, 邹明强. 食品中常见的非食用色素的危害与检测[J]. 中国仪器仪表, 2009, (8): 45-50.

[9] 肖义夫. 食品中非食用色素及其检测方法研究进展[J]. 现代预防医学, 2008, 35(16): 3159-3160, 3164.

[10] N. Yoshioka, K. Ichihashi. Determination of 40 synthetic food colors in drinks and candies by high-performance liquid chro- matography using a short column with photodiode array detec- tion. Talanta, 2008, 74(5): 1408-1413.

[11] 赵延胜, 董英, 张峰等. 食品中46种禁限用合成色素的分级提取净化体系研究[J]. 分析化学, 2012, 40(2): 249-256.

[12] 姜觅, 王恒彦, 汪国权. 液体饮品中20种分散性染料的液相色谱测定研究[J]. 分析测试学报, 2012, 31(9): 1137-1141.

[13] 陈驰. 基于液相色谱—飞行时间质谱建立食品中着色剂筛查方法的研究[D]. 福建农林大学, 2012.

[14] 路勇, 渠岩, 冯楠等. 超高效液相色谱——串联四极杆质谱法快速筛查食品中的45种工业染料[J]. 食品科学, 2012, 33(6): 176-180.

[15] 朱永红, 张庆合, 李根容等. 辣椒制品中大红粉的筛查及检测[J]. 中国调味品, 2011, 36(6): 100-104.

[16] GB/T 5009.36-2003. 食品中合成着色剂的测定[S]. 2003.

[17] GB/T 19681-2005. 食品中苏丹红染料的测定 高效液相色谱法[S]. 2005.

[18] GB/T 23496-2009. 食品中禁用物质的检测 碱性橙染料 高效液相色谱法[S]. 2009.

[19] SN/T 1743-2006. 食品中诱惑红、酸性红、亮蓝、日落黄的含量检测——高效液相色谱法[S]. 2006.

[20] SN/T 2430-2010, 进出口食品中罗丹明B的检测方法[S]. 2010.

[21] DB35/T 897-2009. 食品中碱性橙、碱性嫩黄O和碱性桃红T含量的测定——液相色谱法和液相色谱-串联质谱法[S]. 2009.

[22] DB35/T 896-2009. 食品中碱性桃红T含量的测定 液相色谱-荧光检测法[S].

[23] DBS22/ 008-2012. 食品中酸性橙、碱性橙2和碱性嫩黄的测定——液相色谱–串联质谱法[S]. 2012.

[24] DB33/T 703-2008. 食品和农产品中多种碱性工业染料的测定 液相色谱–串联质谱法[S]. 2008.

[25] 全国打击违法添加非食用物质和滥用食品添加剂专项整治领导小组. 关于印发全国打击违法添加非食用物质和滥用食品添加剂专项整治抽检工作指导原则和方案的通知(食品整治办[2009]29号)[Z], 2009年3月23日.

[26] 赵飞. 食用合成色素及其检测技术的研究进展[J]. 食品安全导刊, 2012, 1-2: 32-33.

[27] 薛虎寅, 尹永梅, 张太昌等. 偶氮类合成色素检测技术的研究进展[J]. 生物技术进展, 2012, 2(3): 171-176.

[28] M. Kucharska, J. Grabka. A review of chromatographic methods for determination of synthetic food dyes. Talanta, 2010, 80(3): 1045-1051.

[29] R. Rebane, I. Leito, S. Yurchenko, et al. A review of analytical techniques for determination of Sudan I-IV dyes in food ma- trixes. Journal of Chromatography A, 2010, 1217(17): 2747- 2757.

[30] 张胜帮, 韩超, 刘继东等. 食品中苏丹红Ⅰ~Ⅳ及对位红的GC-MS/SIM法研究[J]. 中国食品学报, 2009, 9(2): 187-193.

[31] 张玮玮, 刘玲玲, 武彦文等. 食用天然辣椒红色素中掺杂苏丹红的红外光谱快速识别[J]. 光谱学与光谱分析, 2012, 32(4): 958-961. .

[32] 杜一平, 向春兰, 黄子夏等. 辣椒粉掺杂苏丹红的近红外光谱鉴别及其含量测定方法研究[J]. 计算机与应用化学, 2013, 30(1): 36-38.

[33] C. V. Di Anibal, I. Ruisánchez and M. P. Callao. High-resolution 1H Nuclear magnetic resonance spectrometry combined with chemometric treatment to identify adulteration of culi-nary spices with Sudan dyes. Food Chemistry, 2011, 124(3): 1139-1145.

[34] 赵珊, 张晶, 杨奕等. 超高效液相色谱–电喷雾串联四极杆质谱法检测果汁和葡萄酒中的27种工业染料[J]. 色谱, 2010, 28(4): 356-362.

[35] 赵延胜, 杨敏莉, 张峰等. 液相色谱/四极杆–飞行时间质谱法筛查奶酪中29种禁用和限用合成色素[J]. 色谱, 2011, 29(7): 631-636.

[36] 赵海燕, 赵榕, 李兵等. HPLC同时测定调味料中非法添加多组分工业染料的方法研究[J]. 中国食品卫生杂志, 2011, 23(6): 527-531.

[37] 郭新东, 冼燕萍, 罗海英等. 凝胶净化/超高效液相色谱串联质谱法测定调味酱中32种工业染料[J]. 分析测试学报, 2012, 31(6): 658-663.

[38] 李鹏, 李晓丽, 苗虹等. 高效液相色谱–线性离子阱质谱法测定辣椒制品中23种工业染料[J]. 中国食品卫生杂志, 2012, 24(5): 430-435.

[39] F. Feng, Y. S. Zhao, W. Yong, et al. Highly sensitive and accu- rate screening of 40 dyes in soft drinks by liquid chromatogram- phy—electrospray tandem mass spectrometry. Journal of Chro- matography B, 2011, 879(20): 1813-1818.

[40] K. Ma, Y. N. Yang, X. X. Jiang, et al. Simultaneous determination of 20 food additives by high performance liquid chro-matography with photo-diode array detector. Chinese Chemical Letters, 2012, 23: 492-495.

[41] 汪辉, 曹小彦, 李林等. 反相高效液相色谱法对果蔬汁饮料中14种常见食品添加剂的快速测定[J]. 分析测试学报, 2009, 28(10): 1194-1197.

[42] M. Ma, X. B. Luo, B. Chen, et al. Simultaneous determination of water-soluble and fat-soluble synthetic colorants in foodstuff by high-performance liquid chromatogra-phy—Diode array detec- tion—Electrospray mass spectrometry. Jour-nal of Chromatogra- phy A, 2006, 1103(1): 170-176.

[43] 雷雅娟, 徐烨, 刘渭萍等. 高效液相色谱法同时测定食品中的苏丹红、苋菜红和胭脂红[J]. 分析试验室, 2007, 26(4): 85-88

[44] 钟少枢. HPLC法同时测定红油豆瓣酱中的7种非食用色素[J]. 现代食品科技, 2011, 27(8): 1047-1048

[45] USEPA SW-846 method 3640A, Gel-permeation clean-up [S]. revision 1, September 1994.

[46] H.-W. Sun, F.-C. Wang, L.-F. Ai. Determination of banned 10 azo-dyes in hot chilli products by gel permeation chromatogram- phy—Liquid chro-matography—Electrospray ionization-tandem mass spectrometry. Journal of Chromatography A, 2007, 1164(1- 2): 120-128.

[47] O. Pardo, V. Yusà, N. León, et al. Development of a method for the analy-sis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chro- matography with elec-trospray ionization-tandem mass spec- trometry. Talanta, 2009, 78(1), 178-186.

[48] 朱永红, 李根容, 龚迎昆等. HPLC法同时检测辣椒制品中8种非食用红色色素[J]. 中国调味品, 2007, (11): 58-61, 65.

[49] C. Y. Long, Z. B. Mai, X. F. Yang, et al. A new liquid—liquid extraction method for determination of 6 azo-dyes in chilli pro- ducts by high-performance liquid chromatography. Food Chem- istry, 2011, 126(3): 1324-1329.

[50] Y. C. Fan, M. L. Chen, S. T. Chao, et al. Ionic liquids extraction of Para Red and Sudan dyes from chilli powder, chilli oil and food additive combined with high performance liquid chromatography. Analytica Chimica Acta, 2009, 650(1): 65-69.

[51] F. J. López-Jiménez, S. Rubio, D. Pérez-Bendito. Supramolecu- lar sol-vent-based microextraction of Sudan dyes in chilli-con- taining food-stuffs prior to their liquid chromatography-photo- diode array determi-nation. Food Chemistry, 2010, 121(3): 763- 769.

[52] 霍艳敏, 王骏, 张卉等. 高效液相色谱法同时测定冰淇淋中的10种合成色素[J]. 分析测试学报, 2011, 30(6): 670-673.

[53] L. Enríquez-Gabeiras, A. Gallego, R. M. Garcinuño, et al. Inter- ference-free determination of illegal dyes in sauces and condi- ments by matrix solid phase disper-sion (MSPD) and liquid chromatography (HPLC-DAD). Food Chem-istry, 2012, 135(1): 193-198.

[54] 侯晓林, 孙英健, 吴国娟等. 基质固相分散术-UPLC法检测禽蛋中对位红和苏丹红[J]. 食品科学, 2010, 31(24): 285-288.

[55] G. Kesiūnaitė, A. Linkevičiūtė, E. Nau-jalis, et al. Matrix solid- phase dispersion extraction and UPLC deter-mination of sudan dyes in chili powder. Chromatographia, 2009, 70(11-12): 1691- 1695.

[56] AOAC Official Method 2007.01. Pesti-cide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: Gas chromatography/mass spectrometry and liquid chromatog- raphy/tandem mass spectrometry [S]. 2007.

[57] BS EN 15662:2008. Foods of plant origin—Determination of pesticide resi-dues using GC-MS and/or LC-MS/MS following acetonitrile extrac-tion/partitioning and clean-up by dispersive SPE—QuEChERS-method [S]. 2008

[58] 刘丽, 吴青, 林凤英等. QuEChERS-HPLC快速测定食品中七种食用合成色素[URL]. 食品工业科技. 2013. http://www.cnki.net/kcms/detail/11.1759.TS.20130329.1642.002.html

[59] H. Y. Yan, J. D. Qiao, Y. N. Pei, et al. Molecularly imprinted solid-phase extraction coupled to liquid chromatography for determi-nation of Sudan dyes in preserved beancurds. Food Chemistry, 2012, 132(1): 649-654.

[60] C. Y. Long, Z. B. Mai, Y. F. Yang, et al. Synthe-sis and charac- terization of a novel molecularly imprinted polymer for simul- taneous extraction and determination of water-soluble and fat- soluble synthetic colorants in chilli products by solid phase ex- traction and high performance liquid chromatography. Journal of Chromatog-raphy A, 2009, 1216(47): 8379-8385.

[61] M. Ávila, M. Zougagh, A. Escarpa, et al. Determination of sudan dyes in food samples using supercritical fluid extraction—Cap- illary liquid chromatography. The Journal of Supercritical Fluids, 2011, 55(3): 977-982.

[62] Q. G. Liao, W. H. Li, L. G. Luo. Applicability of accelerated solvent extraction for synthetic colorants analysis in meat prod- ucts with ultrahigh perform-ance liquid chromatography—Pho- todiode array detection. Analytica Chimica Acta, 2012, 716: 128-132.

[63] K. S. Minioti, C. F. Sakel-lariou, N. S. Thomaidis. Determination of 13 synthetic food colorants in water-soluble foods by rever- sed-phase high-performance liquid chromatography coupled with diode-array detector. Analytica Chimica Acta, 2007, 583(1): 103-110.

[64] European Commission. NEWS notification: 03/99. Corrected method for the detection of Sudan.

分享
Top