Abstract: Objective: To Build a method (LAMP, Loop mediated isothermal amplification) for detecting Human Bocavirus. Methods: By designing 4 LAMP primers specified for 6 sites in NP1 gene sequence of Bocavirus, we built a system to detect Human Bocavirus. And we detected 60 oropharyngeal swab samples by using this system and realtime fluores-cence quantitative PCR to check whether this system works well at the same time. Results: We found that we can get the production through the LAMP amplification and the gel electrophoresis results of the 60 samples, in which we found 8 positive samples, are consensus between the realtime fluores-cence quantitative PCR and the LAMP system. Conclusion: LAMP, a kind of quick, simple, specific, sensitive and low cost method in detecting the nucleic acids of virus, is an ideal alternative for quick screening assay and clinical detection in medical institutions because of the experimental instruments and requirements are not high.