Study on Content and Antimutagenic Effect of Lentinas Edodes Polysaccharides
>Objective: To analyze the content of polysaccharides in lentinas edodes and investigate the effect on antimutation of polysaccharides, so as to provide the reference for exploitation and utilization of lentinas edodes. Methods: Lentinas edodes polysaccharides were measured by sulfuric acid-phenol test. Amesassay, micronucleus formation assay and testicle cells chromosome aberration test were used to analyze the antimutagenic activities of Lentinas edodes polysaccharides. Results: The content of lentinas edodes polysaccharides ranged from 0.236 mg/g (dry weight) to 0.268 mg/g (dry weight). In four treatment groups (added S9 or not), the lentinas edodes polysaccharides in TA98 and TA100 could effectively inhibit revertant colonies induced by Dexon, MMS and 2-AAF, and the inhibitory rates were 37.7%, 69.8%, 70.3%, 78.1%, 39.0%, 78.1%, 83.0% and 88.6%, respectively. The lentinas edodes polysaccharides could inhibit cyclophosphamide-induced micronucleus formation of polychromatic erythrocytes of mice bone in treatment groups with the inhibitory rate of 31.80%, 52.80% and 63.10%, respectively. The lentinas edodes polysaccharides could markedly reduce the rate of chromosome aberration in mice testicle cells induced by mitomycin C, and the inhibitory rates were 13.9%, 23.3% and 77.9%, respectively. Conclusions: The polysaccharide of lentinas edodes has certain ability of antimutation.
文章引用: 阮海星 , 俞红 , 刘佳 (2013) 香菇多糖成分测定及抗突变作用的研究。 环境保护前沿， 3， 54-58. doi: 10.12677/AEP.2013.31B012
 Chinara. G. 香菇多糖免疫药理作用[J]. 国外医学植物学分册, 1993, 8 ( 4) : 165- 166.
 中国医学科学院药物研究所. 中药有效成分的研究[M] . 北京: 人民出版社, 1972：227-228.
 佘晓雷, 张可, 郑旭霞. 芦荟中多糖含量测定方法的探讨[J] . 营养学报, 2003, 25（2）: 149-152.
 卫生部监督司, 保健食品功能学评价程序和方法[S ] . 1996：63-66.
 赵素娟, 王淑惠, 邓雪, 等. 盐藻素抗突变作用的初探[J].卫生毒理学杂志, 2003, 17: 65-66.
 陈冠敏, 林升清, 黄宗秀, 等. 香菇抗突变作用的研究[J] .癌变、畸变、突变, 2000, 12 ( 2) : 105-108.
 Y. He. Effects of Carotenids on afla-toxin B- induced mutagenesis in Styphimurium TA98 and TA100. Nutr Cancer, 1990, 13（4）: 243- 247