Vol.3 No.2 (March 2013)
Preparation and Lysozyme Adsorption Performance of Meso-structured Cellular Foams with Different Pore Size
The mesostructured cellular foams (MCFs) were synthesized using microemulsion templating, in which the nonionic triblock copolymer surfactant Pluronic P123 was served as template and 1,3,5-trimethylbenzene (TMB) as organic swelling agent. By controlling the mass ratio of TMB/P123, a series of MCFs with different pore size were prepared, and then the structural and chemical properties of MCFs were characterized by TEM and nitrogen adsorption. It was found that the pore size of MCFs increased when the mass ratio of TMB/P123 increased from 0.5 to 1.0, while that of MCFs decreased when the mass ratio continuously increased to 1.5. The MCFs were used as adsorbent for the adsorption of lysozyme. The maximum adsorption rate was obtained on MCFs-1.5 (mass ratio of TMB/P123 was 1.5), meaning that the sample reached equilibrium within 1 h. The immobilization of lysozyme on MCFs-1.5 prevented the leaching of enzyme effectively and the immobilized amount was up to 490 mg/g. Furthermore, FT-IR analysis revealed that the lysozyme adsorbed on MCFs could be held without evident structure changes. This study suggested that enzyme loading efficiency was clearly dependent on the size matching between the enzyme molecules and carrier pores, and the synthesized MCFs could be applied as excellent carriers for enzyme immobilization.
李 俊 , 尹光福 , 丁 艺 (2013) 不同孔径MCFs的制备及其对溶菌酶的吸附性能。 材料科学， 3， 56-60. doi: 10.12677/MS.2013.32011
 C. T. Kresge, M. E. Leonowicz, W. J. Roth, et al. Ordered me-soporous molecular sieves synthesized by a liquid-crystal template mechanism. Nature, 1992, 359: 710-712.
 J. S. Beck, J. C. Vartuli, W. J. Roth, et al. A new family of mesoporous molecular sieves prepared with liquid crystal templates. Journal of the American Chemical Society, 1992, 114(27): 10834-10843.
 P. H. Pandya, R. V. Jasra, B. L. Newalkar, et al. Studies on the activity and stability of immobilized α-amylase in ordered mesoporous silicas. Microporous and Mesoporous Materials, 2005, 77: 67-77.
 W. Chouyyok, J. Panpranot, C. Thanachayanant, et al. Effects of pH and pore characters of mesoporous silicas on horseradish peroxidase immobilization. Journal of Molecular Catalysis B: Enzymatic, 2009, 56: 246-252.
 P. Schmidt-Winkel, W. W. Lukens, D. Zhao, et al. Mesocellular siliceous foams with uniformly sized cells and windows. Journal of the American Chemical Society, 1999, 121: 254-255.
 P. Schmidt-Winkel, W. W. Lukens, P. Yang, et al. Microemulsion templating of siliceous mesostructured cellular foams with well- defined ultralarge mesopores. Chemistry of Materials, 2000, 12: 686-696.
 K. Szymańska, J. Bryjak, J. Mrowiec-Białoń, et al. Application and properties of siliceous mesostructured cellular foams as enzymes carriers to obtain efficient biocatalysts. Microporous and Mesoporous Materials, 2007, 99: 167-175.
 S. Wu, L. Zhang, L. Qi, et al. Ultra-sensitive biosensor based on mesocellular silica foam for organophosphorous pesticide detection. Biosensors and Bioelectronics, 2011, 26: 2864-2869.
 A. Katiyar, L. Ji, P. Smirniotis, et al. Protein adsorption on the mesoporous molecular sieve silicate SBA-15: Effects of pH and pore size. Journal of Chromatography A, 2005, 1069: 119-126.
 S. Hudson, E. Magner, J. Cooney, et al. Methodology for the immobilization of enzymes onto mesoporous materials. Journal of Physical Chemistry B, 2005, 109: 19496-19506.
 M. S. Bhattacharyya, P. Hiwale, M. Piras, et al. Lysozyme adsorption and release from ordered mesoporous materials. Journal of Physical Chemistry C, 2010, 114: 19928-19934.
 J. S. Lettow, Y. J. Han, P. Schmidt-Winkel, et al. Hexagonal to mesocellular foam phase transition in polymer-templated meso- porous silicas. Langmuir, 2000, 16: 8291-8295.
 S. J. Gregg, K. S. W. Sing. Adsorption, surface area and pososity. New York: Academic Press, 1982.
 D. T. Tran, K. J. Balkus. Perspective of recent progress in immobilization of enzymes. Catalysis, 2011, 1: 956-968.
 李霞, 邢向英, 任铁真. 具有大孔–介孔结构的磷酸钛的合成及溶菌酶吸附[J]. 河北工业大学学报, 2012, 41(1): 38-43.
 代文彦, 邹永存, 王海果等. 孔径和比表面积调控对SBA-15上溶菌酶吸附动力学的影响[J]. 吉林大学学报, 2011, 49(1): 139-144.
 Y. Li, G. Zhou, C. Li, et al. Adsorption and catalytic activity of porcine pancreatic lipase on rod-like SBA-15 mesoporous material. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2009, 34: 79-85.