Vol.1 No.1 (July 2011)
High Throughput Sequencing Methods and Applications of Read Mapping Algorithm
Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) has become a tool for studying DNA-binding proteins profile and histone modifications. At the same time, it also arouse requirement for ef-fective computational method to map short DNA reads and to compare mapping profiles, which are crucial for uncovering biological mechanisms. In this article, we introduced the principle of new generation se-quencing and corresponding data format, then give an overview of current method of reads mapping and peak identification. Finally, we demonstrated the application of this method in histone modifications analysis and transcription factor binding sites identification.
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