Vol.9 No.2 (June 2019)
Screening of Endonu-cleases for Identification of A1298C Locus of MTHFR Gene by PCR-RFLP
何震宇 ：广东药科大学生物化学与分子生物学系，广东 广州
The aim is to search restrictive endonuclease with suitable price for the genotyping of A1298C locus of MTHFR gene by PCR-RFLP. According to the sequence of A1298C locus of MTHFR gene in dbSNP database, various on-line restriction site analysis tools were used to select the enzymes that could identify the site, and then through price comparison and analysis of whether there were interference sequences in the upstream and downstream of the polymorphic locus, the candidate enzymes were determined. A cheap enzyme, HinfI, has the potential to identify A1298C locus by PCR primer introduced restriction analysis, which laid a foundation for the joint detection of A1298C locus and C677T locus of MTHFR gene.
何震宇 (2019) 基于PCR-RFLP法鉴定MTHFR基因A1298C位点的内切酶的筛选。 计算生物学， 9， 9-13. doi: 10.12677/HJCB.2019.92002
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