计算生物学

Vol.9 No.2 (June 2019)

基于PCR-RFLP法鉴定MTHFR基因A1298C位点的内切酶的筛选
Screening of Endonu-cleases for Identification of A1298C Locus of MTHFR Gene by PCR-RFLP

 

作者:

何震宇 :广东药科大学生物化学与分子生物学系,广东 广州

 

关键词:

MTHFR基因A1298C位点PCR-RFLP创造酶切位点 Methylenetetrahydrofolate Reductase Gene A1298C Locus Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Created Restriction Site

 

摘要:

寻找价格适宜的限制性内切酶,以期用于PCR-RFLP法对MTHFR基因A1298C位点的分型。根据dbSNP数据库中MTHFR基因A1298C位点的序列,通过各种在线酶切位点分析工具,查找可鉴定该位点的酶,再通过价格对比和分析多态位点上、下游是否存在干扰序列,从而确定候选酶。结合PCR创造酶切位点技术,一种价格便宜的常用酶HinfI具有鉴别该位点的潜力,不仅为该位点的独立检测并且为其与MTHFR基因C677T位点的联合检测奠定了基础。

The aim is to search restrictive endonuclease with suitable price for the genotyping of A1298C locus of
MTHFR gene by PCR-RFLP. According to the sequence of A1298C locus of MTHFR gene in dbSNP database, various on-line restriction site analysis tools were used to select the enzymes that could identify the site, and then through price comparison and analysis of whether there were interference sequences in the upstream and downstream of the polymorphic locus, the candidate enzymes were determined. A cheap enzyme, HinfI, has the potential to identify A1298C locus by PCR primer introduced restriction analysis, which laid a foundation for the joint detection of A1298C locus and C677T locus of MTHFR gene.

文章引用:

何震宇 (2019) 基于PCR-RFLP法鉴定MTHFR基因A1298C位点的内切酶的筛选。 计算生物学, 9, 9-13. doi: 10.12677/HJCB.2019.92002

 

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