分析化学进展

Vol.6 No.3 (August 2016)

不同仪器测定化妆品中氯霉素的含量
Different Instrument for Determining the Content of Chloramphenicol in Cosmetics

 

作者:

陈 科 , 刘翠红 , 李练培 :广东省保化检测中心有限公司,广东 广州

 

关键词:

超高效液相色谱仪二极管阵列检测器祛痘除螨类化妆品氯霉素Ultra High Performance Liquid Chromatograph Diode Array Detector Cosmetics for Removing Acne and Acarus Chloramphenicol

 

摘要:

目的:采用三种不同液相色谱仪,对祛痘除螨类化妆品中氯霉素含量进行测定。方法:在相同的实验条件下,以氯霉素为标准物质,C18柱(250 mm × 4.6 mm, 5 µm)为分离柱,(甲醇 + 乙腈 = 1:2):0.01 mol/L草酸(pH = 2.0) = 40:60为流动相洗脱,二极管阵列检测器,检测波长268 nm,流速:0.8 mL/min。结果:L-3000、LC-20AT、L-6000测定的线性相关系数r分别为0.9976、0.9994、0.9998,精密度的保留时间与峰面积RSD%依次分别为1.8%和2.1%、0.85%和0.91%、0.50%和0.72%,稳定性RSD%分别为2.3%、0.37%、0.16%,重复性RSD%分别为2.7%、0.86%、0.57%,回收率和RSD%依次分别为96.2%、2.2%;97.4%、1.3%;99.3%、0.7%。结论:经比较,使用超高效液相色谱仪测定氯霉素灵敏度低、精密度高、重复性好。

Objective: Using three different liquid chromatographs, to determine the chloramphenicol content in cosmetics for removing acne and acarus. Methods: Under the same experimental conditions, on the basis of chloramphenicol material, C18 column (250 mm × 4.6 mm, 5 µm) was used as the sep-aration column, (methanol + acetonitrile = 1:2):0.01 mol/L oxalic acid (pH = 2.0) = 40:60 as mobile phase elution, and diode array detector was also used with detection wavelength of 268 nm, velocity of 0.8 mL/min. Results: The linear correlation coefficient r of L-3000, LC-20AT, L-6000 was 0.9976, 0.9994, 0.9998 respectively; RSD% of precision retention time and peak area were 1.8% and 2.1%, 1.8% and 0.91%, 0.50% and 0.72%, respectively; RSD% of stability was 2.3%, 0.37% and 2.3% respectively; RSD% of repetitive peak area was 2.7%, 0.86% and 2.7% respectively; the recovery rate and RSD% were 96.2% and 96.2%, 97.4% and 1.3%, 99.3% and 0.7% respectively. Conclusion: After comparing, using ultra performance liquid chromatography to determine chloramphenicol has advantages of low sensitivity, high precision and good repeatability.

文章引用:

陈 科 , 刘翠红 , 李练培 (2016) 不同仪器测定化妆品中氯霉素的含量。 分析化学进展, 6, 56-61. doi: 10.12677/AAC.2016.63009

 

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