Vol.6 No.4 (July 2016)
Preparation of BMP-2-PLLA Microspheres and Its Maintenance for Bioactivity of BMP-2
The preparation conditions of poly-l-lactide (PLLA) microspheres, release characteristics and bi-oactivity of Bone Morphogenetic Protein-2 (BMP-2) were studied. Bovine serum albumin (BSA)- loaded PLLA microspheres were prepared with a double emulsion method. The effects of the mo-lecular weight and concentration of PLLA, the molecular weight of PEG on the particle size, loading capacity and entrapment efficiency were studied. Then, the optimal conditions were selected to prepare BMP-2-loaded PLLA microspheres, and the bioactivity of BMP-2 was investigated by pro-duction of cell Alkaline Phosphatase (ALP). The results showed that the BMP-2-PLLA microspheres were spherical shape with diameters of 4.815 μm. The encapsulation efficiency of the BMP-2 in PLLA microspheres was 70.88%, and the loading capacity was 2.28 × 10−3%. The residual amount of methylene chloride in BMP-2-PLLA microsphere was 0.0041%. BMP-2-loaded PLLA microspheres had excellent drug sustained-release performance, which would control release BMP-2. BMP-2 biological activity indicated that the biological activity was not effected after emulsion process. The bioactivity of growth factors released from PLLA microspheres was more durable and effective. PLLA microspheres prepared can be used to load growth factors to control their release and maintain their biological activity.
白 燕 , 肖 唯 (2016) BMP-2-PLLA缓释微球的制备及其对BMP-2活性的维持。 材料科学， 6， 268-275. doi: 10.12677/MS.2016.64035
 Del, R.C., Rodrguez-Évora, M., Reyes, R., et al. (2015) BMP-2, PDGF-BB, and Bone Marrow Mesenchymal Cells in a Macroporous β-TCP Scaffold for Critical-Size Bone Defect Repair in Rats. Biomedical Materials, 10, 045008.
Buchmann, S., Sandmann, G.H., Walz, L., et al. (2015) Growth Factor Release by Vesicular Phospholipid Gels: In- Vitro Results and Application for Rotator Cuff Repair in a Rat Model. BMC Musculoskeletal Disorders, 16, 1-10.
Qi, Z.R., Zhang, Q., Tan, L.L., et al. (2014) Comparison of Degradation Behavior and the Associated Bone Response of ZK60 and PLLA in Vivo. Journal of Biomedical Materials Research Part A, 102, 1255-1263.
Bai, Y., Yin, G.F., Huang, Z.B., et al. (2013) Localized Delivery of Growth Factors for Angiogenesis and Osteogenesis in Tissue Engineering. International Immunopharmacology, 2, 214-223.
Han, F., Zhou, F., Yang, X., et al. (2014) Facile Preparation of PLGA Mi-crospheres with Diverse Internal Structures by Modified Double-Emulsion Method for Controlled Release. Polymer Engineering & Science, 55, 896-906.
Polo-Corrales, L., Latorre-Esteves, M. and Ramirez-Vick, J.E. (2014) Scaffold Design for Bone Regeneration. Journal of Nanoscience & Nanotechnology, 14, 15-56.
 马爱洁, 张玉祥, 陈卫星. 复相乳液法制备聚乳酸/胰岛素缓释微胶囊[J]. 西安工业大学学报, 2009, 29(4): 341- 344.
 张万国, 胡晋红, 蒋雪涛, 等. 聚乳酸相对分子量对利福平微球及其药物分布状态的影响[J]. 解放军药学学报, 2001,17(2): 59-61.
 Maekawa, T. (2011) Polymeric Scaffolds in Tissue Engineering Application: A Review. International Journal of Polymer Science, 2011, 609-618.
 李素铠, 欧阳斌, 徐晓秋, 等. PLGA纳米药物递送系统的建立及其对bFGF生物学活性的维持[J]. 第三军医大学学报, 2016, 38(11): 1319-1324.