Vol.6 No.4 (July 2016)
Preparation and Release Performance in Vitro of Chitosan Microspheres/nHA/PLGA Compound Scaffolds
In order to construct a bone regeneration scaffold that different growth factors controlled release at proper stage, Trypsin (Try) was selected as the model protein. The composite of sustained-re- lease microspheres and biodegradable porous scaffolds was prepared in this study. Firstly, Tryp-sin-chitosan microspheres (CMs) were prepared. Then, Trypsin-CMs were compounded to the nano hydroxyapatite/poly lactic-co-glycolic acid (nHA/PLGA) to build a scaffold that could release growth factors sequentially. The results showed that the Try-CMs were spherical shape with diameters of 4 - 10 μm. The encapsulation efficiency of the Trypsin in CMs was 61.33%, and the loading capacity was 25.699%. The prepared Try-CMs/nHA/PLGA scaffold possessed 100 - 200 μm pore diameter, 53.24% porosity, 7.31 MPa compressive strength, and 19.92% degradation at 8 weeks. The cumulative releases of Trypsin from Try-nHA/PLGA, Try-CMs and Try-CMs/nHA/PLGA scaffolds were respectively about 57.31%, 69.32% and 26.03% at 48 hours, 77.89%, 85.73% and 54.53% at 14 days. The results demonstrated that Try-CMs/nHA/PLGA scaffolds had excellent drug sustained-release performance, which would be used as tissue engineering scaffolds with protein controlled delivery.
白 燕 , 肖 唯 (2016) CMs/nHA/PLGA复合支架的制备及体外释放性能的研究。 材料科学， 6， 256-262. doi: 10.12677/MS.2016.64033
Deschaseaux, F., Sensébé, L. and Heymann, D. (2009) Mechanisms of Bone Repair and Regeneration. Trends in Molecular Medicine, 15, 417-429.
Nakano, K., Murata, K., Omokawa, S., et al. (2016) Promo-tion of Osteogenesis and Angiogenesis in Vascularized Tissue-Engineered Bone Using Osteogenic Matrix Cell Sheets. Plastic & Reconstructive Surgery, 137, 1476-1484.
Kanczler, J.M., Ginty, P.J., White, L., et al. (2010) The Effect of the Delivery of Vascular Endothelial Growth Factor and Bone Morphogenic Protein-2 to Osteoprogenitor Cell Populations on Bone Formation. Biomaterials, 31, 1242- 1250.
 Wang, Q., Hua, Q. and Wang, S. (2007) Application of Fibrin Glue in Facial Nerve Repair. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi, 24, 612-614.
Thevenot, P.T., Nair, A.M., Shen, J.H., Lotfi, P., Ko, C.-Y. and Tang, L.P. (2010) The Effect of Incorporation of SDF- 1α into PLGA Scaffolds on Stem Cell Recruitment and the Inflammatory Response. Biomaterials, 31, 3997-4008.
Ambrosio, A.M., Sahota, J.S. and Khan, Y. (2001) A Novel Amor-phous Calcium Phosphate Polymer Ceramic for Bone Repair: I. Synthesis and Characterization. Journal of Biomedical Materials Research, 58, 295-301.
 赵君, 蒋欣泉, 张志愿. 羟磷灰石/聚乙丙交酯生物复合材料的研究进展[J]. 国际口腔医学杂志, 2008, 35(2): 207- 209.
 安世昌, 孙健, 李亚莉等. 复合壳聚糖纳米微球聚乳酸–羟基乙酸/纳米羟基磷灰石缓释载体系统对蛋白的缓释作用[J]. 中国组织工程研究与临床康复, 2011, 15(25): 4615-4618.
Bai, Y., Yin, G.F., Huang, Z.B., et al. (2013) Localized Delivery of Growth Factors for Angiogenesis and Osteogenesis in Tissue Engineering. International Immunopharmacology, 2, 214-223.
 Maekawa, T. (2011) Polymeric Scaffolds in Tissue Engineering Application: A Review. International Journal of Polymer Science, 2011, 609-618.
Sun, Y., Wang, J., Feng, Z., et al. (2016) The Preparation and Characteristics of Spray Dried Astragalus Polysaccharides/Chitosan Microspheres for Pulmonary Drug Delivery. Nanomedicine Nanotechnology Biology & Medicine, 12, 565-565.
Morille, M., Toupet, K., Montero-Menei, C.N., et al. (2016) PLGA-Based Microcarriers Induce Mesenchymal Stem Cell Chondrogenesis and Stimulate Cartilage Repair in Osteoarthritis. Biomaterials, 88, 60-69.
Cui, H.X., Guo, J., Han, Z., et al. (2015) Biocompatibility of Differ-ently Proportioned HA/PLGA/BMP-2 Composite Biomaterials in Rabbits. Genetics & Molecular Research GMR, 14, 13511-13518.