药物资讯

Vol.4 No.4 (November 2015)

HPLC测定扶正柔肝颗粒柯里拉京和丹酚酸B的含量
Determination of Corilagin and Salvianolic Acid B in Fu Zheng Rou Gan by HPLC

 

作者:

宋力飞 , 刘常青 , 李曼莎 :广州泽力医药科技有限公司,广东 广州

阳建军 , 李常青 :广州中泽生物技术有限公司,广东 广州

 

关键词:

扶正柔肝颗粒含量测定柯里拉京丹酚酸BHPLCFu Zheng Rou Gan Content Determination Corilagin Salvianolic Acid B HPLC

 

摘要:

目的:建立高效液相色谱法(HPLC)测定复方扶正柔肝颗粒中柯里拉京和丹酚酸B含量的方法。方法:色谱柱:InertsiL® ODS-SP (5 μm, 4.6 × 250 mm);流动相:乙腈-0.2%磷酸(梯度洗脱);检测波长为210 nm;流速为1.0 mL/min;柱温35℃;进样量5 μL。结果:柯里拉京在0.061~0.510 μg的范围内线性关系良好(r = 0.9997),平均加样回收率为101.41% (RSD = 1.68%, n = 6);丹酚酸B在0.504~4.203 μg的范围内(r = 0.9999)线性关系良好,平均加样回收率为103.30% (RSD = 1.11%, n = 6)。结论:本法灵敏、快速、准确,可用于复方扶正柔肝颗粒的质量控制。

Objective: To establish a high liquid chromatography method of determinating Corilagin and Sal-vianolic acid B in Fu Zheng Rou Gan. Method: Chromatographic column: InertsiL® ODS-SP (5 microns, 4.6 × 250 mm). The mobile phaseis is acetonitrile-0.2% phosphoric acid (gradient elution) with detection wavelength 210 nm; the flow rate is 1.0 mL/min; the column temperature is 35˚C and the inject volume is 5 μL. Result: In the range of 0.061 - 0.510 μg, the linear relationship of Corilagin is good (r = 0.9997), and the average sample recovery rate is 101.41% (RSD = 1.68%, n = 6). In the range of 0.504 - 4.203μg, the linear relationship of Salvianolic acid B is also good (r = 0.9999), and the average sample recovery rate is 103.30% (RSD = 1.11%, n = 6). Conclusion: The method is sensitive, rapid and accurate and can be used for quality control of this granule.

文章引用:

宋力飞 , 刘常青 , 李曼莎 , 阳建军 , 李常青 (2015) HPLC测定扶正柔肝颗粒柯里拉京和丹酚酸B的含量。 药物资讯, 4, 43-49. doi: 10.12677/PI.2015.44006

 

参考文献

分享
Top