分析化学进展

Vol.5 No.3 (August 2015)

美洲大蠊药材HPLC指纹图谱研究
Fingerprint of Periplaneta americana by HPLC

 

作者:

吴红梅 , 杨 烨 , 王祥培 :贵阳中医学院,贵阳

刘李梅 , 杨 明 :成都中医药大学中药材标准化教育部重点实验室,成都

 

关键词:

美洲大蠊药材特征指纹图谱HPLCPeriplaneta americana Characteristic Chromatographic Profile HPLC

 

摘要:

采用Diamonsil-C18(1)色谱柱(250 mm × 4.6 mm, 5 μm),流动相乙腈-0.1%三氟乙酸水溶液,检测波长256 nm,结果10批不同来源的美洲大蠊药材标定了22个共有峰,其中指认了3个共有峰,其相似度大于0.950;与美洲大蠊药材对照指纹图谱比较,1批黑胸大蠊药材的相似度为0.990,但只有18个共有峰。本文建立的方法具有简便,重复性好,特征性强的特点,可用于美洲大蠊药材的鉴别与质量评价。

Taking Diamonsil C18(1) column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.1% trifluoroacetic acid as mobile phase, detection wavelength 256 nm, we got a result that there were 22 common peaks in batches of Periplaneta Americana, the peaks similarity was more than 0.950, three com-mon peaks were identified, the similarity of Periplaneta fulginosa was 0.990 by comparing with the control fingerprint, but there were 18 common peaks. The method is repeatable and feasible and can be suitable for the quality control of Periplaneta americana.

文章引用:

吴红梅 , 杨 烨 , 刘李梅 , 杨 明 , 王祥培 (2015) 美洲大蠊药材HPLC指纹图谱研究。 分析化学进展, 5, 39-48. doi: 10.12677/AAC.2015.53005

 

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