Vol.5 No.3 (July 2015)
Differentiation Ability of Periodontal Ligament Stem Cells in Different Scaffolds
常秀梅 ：广东医学院，广东 东莞
金 岩 ：第四军医大学口腔医学院口腔组织病理教研室，陕西 西安
Objective: To investigate the performance of combine collagen gen and ceramic bovine bone for human periodontal ligament stem cells. Methods: Human periodontal ligament stem cells were cultured in vitro. The subsequent cell passaging was conducted in conditioned medium containing dexamethasone, beta-sodium glycerophosphate and ascorbid acid. Then the human periodontal ligament stem cells were divided into two groups: one was cultured in BBC and the other was cul-tured in LCM. Attachment was observed by SEM and proliferation was evaluated by cell counting, ALP was measured in vitro histochemical method, and differentiation was observed by HE stain after transplant into nude mice. Results: BBC improved the proliferation and differentiation of cells cultured in vitro, showing no difference in attachment between two groups. Conclusion: BBC can improve the proliferation and differentiation of human periodontal stem cells.
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