Vol.5 No.2 (June 2015)
Xuebijing Regulate the Hypothalamic-Pituitary-Adrenal Axis and the Level of GRmRNA of Adrenal in Septic Rats
刘淑正 ：唐山市第二医院重症医学科，河北 唐山
Objective: To investigate the change of CORT and the level of GRmRNA of adrenal in CLP induced septic rats which were treated by Xuebijing, identify that Xuebijing can regulate the CORT and GRmRNA. Methods: 40 male rats were divided randomly into normal control group, sham group, saline group (Sepsis group), Xuebijing for treatment group. The model of sepsis was made by CLP. We observe the clinical manifestation of animal after CLP. We use the radio-immunity method to detect the levels of CORT; using the RT-PCR method to measure the level of GRmRNA in adrenal. Results: In Sepsis group, the plasma CORT concentration (29.74 ± 1.65 ng/ml) was significantly higher than that in the normal control group (7.56 ± 0.89 ng/ml, P < 0.01) and Sham group (13.27 ± 1.31 ng/ml, P < 0.01). In XBJ group, plasma CORT concentration (19.27 ± 1.85 ng/ml) was significantly lower than that in Sepsis group (P < 0.01). There was no significant difference between Normal control and Sham group (P > 0.05). In Sepsis group, the level of the GRmRNA in the adrenal tissue (0.80 ± 0.11) was significantly lower than that in the normal control group (1.44 ± 0.07, P < 0.01) and the Sham group (1.15 ± 0.11, P < 0.01). There was no significant difference between XBJ (1.08 ± 0.10) and Sepsis group (P > 0.05). Conclusion: In septic rats, the HPA axis was excessive activate. Xuebijing can decrease the level of CORT, increase the level of GRmRNA in adrenal, improve the excessive activate condition of HPA axis, and lessen the clinical manifestation.
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