Vol.5 No.1 (March 2015)
Research the Cytotoxicity of Docetaxel Combined with Carboplatin in Osteosarcoma Cell Line MG-63
Objective: To research the cytotoxicity of docetaxel combined with carboplatin on osteosarcoma cell line MG-63. Methods: Using MTT assay, we obtained the mean IC50 of docetaxel and caboplatin for the osteosarcoma cell line MG-63. The MG-63 was incubated with IC50 docetaxel, IC50 carboplatin and 1/2 IC50 docetaxel + 1/2 IC50 carboplatin for 24 h, 48 h, 72 h. The cytotoxicity of each group was measured by MTT assays. Result: By the formula IC50 = lg − 1[Xm − i(ΣP − 0.5)] to obtain the IC50 of docetaxel and carboplatin for osteosarcoma cell MG-63 were 0.25 mg/ml and 4.00 mg/ml respectively. The mean cytotoxicity index (CI) of 0.25 mg/ml docetaxel, 2 mg/ml carboplatin and 0.125 mg/ml docetaxel + 2 mg/ml carboplatin for 24 h were 38.6%, 48.7% and 60.0% respectively (P < 0.05); and those for 48 h were 44.3%, 49.2% and 73.0% respectively (P < 0.05); and those for 72 h were 59.3%, 58.6% and 79.9% respectively (the 0.25 mg/ml docetaxel group and 2 mg/ml carboplatin group P > 0.05; the combined group compared with the other two groups P < 0.05). The cytotoxicity index of combined group was significant increased. Conclusion: The combination of half dose docetaxel with carboplatin appears more active as docetaxel or carboplatin alone in full doses in treatment of osteosarcoma cell line MG-63 in vitro.
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