Vol.4 No.3 (August 2014)
Determination Fluorescence Emission Spectra of Phenazine 5,10-Dioxide Using Multi-Peaks Gaussian Fitting Method in Different Solvent
Phenazine 5,10-dioxide has been synthesized by a reaction of phenazine with hydrogen peroxide (H2O2) in acetic acid (HAc) solution. And its fluorescence emission spectra has been determined in different solvent by overlap peaks of a principal peak with a wavelength (λ) at 520 - 545 nm and a shoulder peak with λ at 500 - 515 nm, which are respectively assigned to dimer and monomer emission peaks. The maximum wavelength, peak area, and other spectral parameters for phenazine 5,10-dioxide dimer and monomer were obtained from the analysis of overlap peaks by a mul-ti-peaks Gaussian fitting method, then the value of relative integral emission intensity for dimer and monomer (I2/I1) in different solvents with various concentration can be obtained. When the concentration of phenazine 5,10-dioxide is low, the value of I2/I1 grows linearly with the concen-tration increase, and the maximum emission peaks of the monomer and dimer redly shift signifi-cantly. When the concentration of phenazine 5,10-dioxide is high, the value of I2/I1 grows with the concentration increase in tetrahydrofuran (THF), ethyl acetate, acetonitrile and ethanol, however it decreases with the concentration increase in benzene, toluene and acetic acid (HAc).
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