Vol.3 No.6 (November 2013)
The Cytocompatibility of Silk Fibroin/Poly(ε-caprolactone) Electrospun Fibers
>In order to develop a new degradable biomaterial with both good biocompatibility and mechanical pro- perties, blended fibers composed of silk fibroin (SF) and poly(ε-caprolactone) (PCL) were fabricated by electrospining technique in this study. Confocal laser scanning microscope, scanning electron microscopy, cell adhesion rate and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay were used to analyse the cytocompatibility of SF/PCL electrospun fibers, and to study the influence of the SF/PCL blend ratio on L-929 cell adhere and proliferate. The results showed that cells on the SF/PCL electrospun fibers surface can adhere and proliferate, and the cell number increased with the prolongation of the culturing time. The rate of cell adhesion and proliferation cultured on the SF/PCL electrospun fibers with the ratio of 70/30 and 50/50 was higher than other SF/PCL blend ratio electrospun fibers.
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